60 



BULLETIN 380^ U. S. DEPARTMENT OF AGEICULTUEE. 



1 inch or less in diameter, was cut off about 6 inches from the 

 main trunk. Mycelium from corn meal in flasks was placed on the 

 cut end of the stub and covered with wet cotton, over which oiled 

 paper was tied. In about two weeks the paper and cotton were re- 

 moved. In all cases, branches similar to those inoculated were cut 

 as checks. 



Tablk III. — Inoculations ivith Endothia gyrosa. 



Source of fungus and 

 date. 



FagLis: 



May 8, 1913 . . . 

 May 29, 1913.. 



Sept. 15, 1913. 



Do 



Apr. 2, 1914 . 

 Do 



Do 



May 23, 1914 . 



Do 



Do , 



Do....'... 

 Quercus: 



May 29, 1913 . 

 Do , 



Sept. 15, 1913. 

 Do....... 



Apr. 2, 1914 . 



Do 



Do 



May 23, 1914 . 



Do 



Do 



Do 



Castanea: 



May 29, 1913 . 

 "Do 



Apr. 2, 1914 . 

 Do 



Do. 



May 23, 1914 . 



Do 



Do 



Liquidambar: 

 May 29, 1913.. 

 Do 



Sept. 15, 1913. 



Do. 



Do 



Apr. 2, 1914 



Do 



Do 



Host inocu- 

 lated. 



Castanea . 

 Fagus 



Liquldambar 

 Quercus 



Fagus — 

 Quercus.. 

 Castanea . 



do 



Liquldambar 



Quercus i 



Fagus 



....do 



Liquldambar 



do... 



Castanea . 



Fagus 



Quercus i . 

 Castanea . . 



.do. 



Liqiudambar 



Quercus ' 



Fagus 



....do 



Liquldambar 



Fagus... 

 Quercus ' 



Castanea . 

 do... 



Liquldambar 

 Quercus i 



Fagus 



Liquldambar 

 Fagus 



Castanea . 



Quercus.. 



Fagus 



Quercus i 

 Castanea . 



May 23J 19iV.".' '.'.'.'.'.\ do 



Do 

 Do 

 Do 



Liquldambar 



Quercus i 



Fagus 



Number 

 of inocu- 

 lations. 



Number 

 success- 

 ful. 



Remarks. 



Pycnospores first observed on Oct. 16. 

 Pyenospores first ol>served on Aug. 29 for 



two and on Oct. 10 tor the third. 

 No growth until the spring of 1914; pyc- 



nidia scattered and small on Oct. 13. 

 No growth until spring; well developed on 



Oct. 13, 1914. 



Pycnidial stromata well developed on Oct. 

 13, 1914. 

 Do. 



Do. 



Pycnospores first observed on Aug. 29, 1913. 

 Very shght indications of growth on Aug. 



29, 1913; a few pycnidia with spores on 



Oct. 16. 



Large well-developed pycnidia on Oct. 13, 

 1914. 



Large abundant pycnidial stromata on Oct. 

 13, 1914. 



Abundant well-developed pycnidial stro- 

 mata on Oct. 13, 1914. 



Pycnospores first observed on Aug. 29, 1913. 

 SUght indications of pycnidial formation on 



Aug. 29, 1913; pycnospores on all on Nov. 



17, 1913. 



Large well-developed pycnidial stromata 



on Oct. 13, 1914. 

 Scattered, fairlv well-developed pycnidia 



on Oct. 13, 1914. 

 Abundant well-developed pycnidia on Oct. 



13, 1914. 



Pycnospores first observed on Aug. 29. 



No evidence of growth until the spring of 

 1914; pycnidia few and small on Oct. IS. 



No growth until the spring of 1914; pyc- 

 nidia small on Oct. 13. 



Abundant pycnidia on Oct. 13, 1914. 



1 The species used in this case was Quercus prinus, which proved to be an exceedingly unfavorable host 

 for Endothia gyrosa. 



