68 



BULLETIN 380, U. S. DEPARTMENT OF AGEICULTURE. 



22 in this locality on Acer rubrum, Carya glabra, Cornus florida, 

 Fagus grandifolia, Liriodendron tulipifera, Quercus prmws, Sas- 

 safras variifolium, Vaccinium sp., and Vitis sp. without success. 

 On April 18, the following hosts were inoculated in Virginia : Acer 

 ruhrum, Betula nigra, Benzoin aestivale, Carpinus caroUniana, Carya 

 glabra, Cornus -florida, Fagus grandifolia, Liriodendron tvllpifera. 

 Prunus serotina, Quercus alba, Ulmus americana, and Vitis sp. 

 Each host was inoculated in from four to six places. Of these, 

 pycnidia were produced only on Acer rubrum, Carpinus, and Lirio- 

 dendron. A similar series was made on the same hosts in the same 

 place on May 27. Inoculations on one tree of Quercus alba showed 

 undoubted evidence of parasitism and is described below. 



On July 9 and 11 from five to fourteen inoculations were made 

 on each of the following hosts at Woodstock, N. Y. : Acer rubrum, 

 Betula alba, Carya ovata, Fagus grandifolia, Fraxinus americana, 

 Hamamelis virginiana, Juglans cinereo., Kalmla latifolia, Nyssa syl- 

 vatica, Ostrya virginiana, Populus grandidentata, Prunus serotina, 

 Rhus typhina, Quercus rubra, Salix sp., Sambucus canadensis, and 

 Sassafras variifolium. Pycnidia appeared on Acer rubrum and 

 Ostrya only. The fungus made considerable growth on two plants 

 of Rhus typhina, partly girdling branches one-half inch in diameter 

 and producing distinct fans. The fans were, however, much smaller 

 than those usually found in Castanea. Inoculations were made at 

 Avon, Conn., July 15, on Acer saccharum, Betula cdba, Carya glabra, 

 Cornus florida, and Ostrya virginiana. Pycnidia developed only 

 on Ostrya. The successful inoculations with Endothia paralitica 

 are shown in Table VII. 



Table YII. — Successful inoculations in IDlJf ivith EnclotMa parasitica on hosts 



other than Castanea. 



Locality. 



Date. 



Host. 



Number 

 of inocu- 

 lations. 



Number 

 success- 

 ful.! 



Virginia.... 



Do 



Do 



Do 



New York.. 

 Do 



ConBecticut. 



18 



Apr. 

 ...do.... 

 ...do.... 

 May 27 

 July 11 

 ...do.... 

 July 15 



Acer rubrum 



Carpinus caroliniana . . . 

 Liriodendron tulipifera . 



Quercus alba 



Acer permsylvanicum. . 



Ostrya virgiaiana 



do 



1 Inoculations producing pycnidia are classed as successful. 



It must be noted that while pycnidia were produced in the cases 

 listed as successful, there was no indication of parasitism, nor did 

 the growth extend beyond the tissue injured by the cut except in 

 Quercus and Rhus. 



Out of about 400 inoculations with Endothia parasitica on hosts 

 other than Castanea, about 70 of which were made on different 



