8 



BULLETIN" 306, U. S. DEPARTMENT OE AGRICULTURE. 



Table IV. — Alkaloids in the leaves of first-generation belladonna plants from close polli- 

 nation and cross-pollination of selected parents at two stages of growth at Arlington, 

 Va., during the first season, 1913 — Continued. 



Summary or Averages for the Various Lots. 



Lot. 



Lot 6w, close pollinated. 

 Lot 6w, cross-pollinated. 

 Lot 7w, close pollinated. 

 Lot 7w, cross-pollinated. 

 Lot 23, cross-pollinated.. 

 Lot 46, cross-pollinated.. 



Alkaloidal 

 yield of 

 parent. 



High. 

 ...do. 

 ...do. 

 ...do- 

 Low. 

 ...do.. 



Average alkaloidal content 

 (per cent) . 



First 

 stage. 



0.672 

 .586 

 .595 

 .618 

 .408 

 .407 



Second 

 stage. 



0.701 

 .602 

 .632 

 .611 



0.686 

 .594 

 .614 

 .615 



The following year, 1914, these plants were again analyzed. The 

 first picking was made on August 1, when the berries were be- 

 ginning to ripen. This is the stage at which, as shown by other 

 experiments, the greatest percentage of alkaloids is present. The 

 second picking was made on September 5, when the berries had all 

 ripened. Table V shows the percentage of alkaloids in each case, 

 the averages being summarized at the close. 



Table V. — Alkaloids in the leaves of first-generation belladonna plants from close polli- 

 nation and cross-pollination of selected parents at two stages of growth at Arlington, 

 Va., during the second season, 1914- 





Alkaloidal content (per cent). 



Lot and number. 



Alkaloidal content (per cent). 



Lot and number. 



First 

 stage, 

 Aug. 1. 



Second 



stage, 



Sept. 5. 



Average. 



First 

 stage, 

 Aug. 1. 



Second 

 stage, 

 Sept. 5. 



Average. 



Lot 6w, close polli- 

 nated: 

 1 



0.864 

 .770 

 .746 

 .858 

 .771 

 .761 



0.882 

 .611 

 .581 

 .858 

 .829 

 .956 

 .924 

 .854 

 .864 

 .618 



0.873 

 .690 

 .663 

 .858 

 .800 

 .858 



Lot 6w, close polli- 

 nated — Contd. 

 31 



1.030 



.902 



1.031 



1.096 



1.043 



.860 



.744 



0.783 

 .792 



0.906 



2 



32 



33 



.847 



3 





4 



34 



.908 

 .765 

 .657 

 .454 



1.002 



5 



35 



.904 



6 



36 



.908 



7 



37 



.549 





.924 

 .916 

 .859 



.889 

 .890 

 .738 



Average 



Lot 6w, cross-polli- 

 nated: 



1 





9 



.906 



.765 









11 





.753 

 .687 

 .717 





12 



1.045 



.953 



.831 



.999 





13 





14... 







2 



1.169 

 .969 



.923 



15... 



.646 



.693 



.669 



3 



.843 



16 



4 





17 



.739 

 .943 

 .930 

 .681 

 .935 

 1.016 



.795 

 .612 

 .708 

 .882 

 .813 

 .747 

 .777 

 .753 

 .837 

 .833 

 .699 

 .691 

 .599 

 .742 



.767 

 .627 

 .819 

 .781 

 .874 

 .881 



5 



1.074 

 .990 

 .987 



.756 

 .575 

 .530 



.915 



18 . 



6 



.782 



19 



7 



.758 



20 



8 





21 



9 



.790 

 .804 

 1.062 

 .771 

 .943 

 .903 

 .810 



.565 

 .663 

 .647 

 .712 

 .626 

 .572 

 .657 



.677 



22 



10 



11 



.733 



23 



.854 



24 



1.193 

 .969 

 .990 



1.103 

 .976 

 .797 

 .941 



.973 

 .905 

 .911 



.856 

 .833 

 .698 

 .841 



12 



.741 



25 



13 



14 



15 



16 



.784 



26 



.737 



27 



.733 



28 





29 



17... 



.929 

 .728 







30 



18 



.621 



.674 



