12 



BULLETIN 306,, TJ. S. DEPABTMENT OF AGEICULTUEE. 



When the plants were about 3 inches high they were divided into 

 three lots. One lot was transplanted to the gardens at Arlington, 

 Va., another lot was shipped to Timmonsville, S. C, and a third lot to 

 Madison, Wis. The object was to grow at these widely separated 

 locations under different soil and climatic conditions plants which 

 were obtained from a definite source. Data would thus be secured 

 which would serve to indicate whether the transmissibility of the 

 alkaloid -producing quality would be affected by external influences. 

 Furthermore, it would throw some light on the question of alkaloid 

 production as dependent on sunlight, rainfall, and soil conditions, 

 which is a question still unsettled. At Arlington and at Madison two 

 pickings were made of all the individuals, while at Timmonsville only 

 one collective sample was secured from the plants from each individual 

 parent. In Table VI the percentage of alkaloids in the individual 

 plants at Arlington and Madison is presented, the summary of averages 

 and the results from Timmonsville being added at the close. 



Table VI. — Alkaloids in the leaves of second-generation belladonna plants from cross- 

 pollinated parents at two stages of growth at Arlington, Va., and Madison, Wis., in 

 1914. 





Arlington, Va. 



Madison, W 



is. 



Lot and number. 



First 



stage, 



Aug. 18. 



Second 



stage, 



Sept. 12. 



Average. 



.First 

 stage, 

 Aug. 4. 



Second 



stage, 



Sept. 5. 



Average. 



Lot 6w 7 : 



1 



0.783 

 .542 



0.461 

 .570 

 .488 

 .666 

 .558 

 .851 

 .620 

 .646 

 .835 

 .653 

 .522 

 .340 

 .605 

 .669 

 .525 



.541 

 .691 

 .516 



0.622 

 .556 

 .488 

 .728 

 .486 

 .851 

 .597 

 .681 

 .836 

 .603 

 .615 

 .557 

 .650 

 .628 

 .525 



.605 

 .723 



.577 



0.962 



.707 



0.956 

 .813 

 .781 

 .653 

 .530 

 .707 

 .791 

 .861 

 .583 

 .461 

 .727 



1.027 

 .813 

 .908 

 .949 



.557 



0.959 



2 



.760 



3 



.781 



4 



.791 

 .414 



.653 

 .791 

 .765 

 .580 

 .599 

 .794 

 .478 

 1.020 

 1.322 

 .867 

 .956 

 .538 



.736 

 .653 

 .685 

 .806 

 .822 

 .762 

 .740 

 .803 

 1.062 

 .695 

 .905 

 .752 

 .775 

 .554 

 .806 



.554 



.573 

 .580 

 .781 

 .630 



.653 



5 



.710 



6 



.736 



7 



.574 

 .716 

 .837 

 .554 

 .708 

 .674 

 .695 

 .588 



.685 



8 



.730 



9 



.688 



10 



.470 



11 



.873 



12 



1.174 



13 



.840 



14 



.932 



15 



.793 



Lot 6wio: 



1 



.669 

 .755 

 .639 



.646 



2 



.653 



3 



.829 

 .759 



1.068 

 .876 

 .813 

 .733 

 .908 

 .838 



1.115 

 .813 

 .943 

 .605 

 .752 



.488 



.757 



4 



.783 





.641 

 .550 



.748 

 .557 



.573 

 .541 



.607 

 .546 

 .748 

 .557 



.945 



6 



.819 



7 



.776 



8 





.768 



9 





.985 



10 



.327 



.398 



.362 



.766 



u...; 



1.010 



12 









.783 



13 : 









.859 



14 









.579 



15 









.779 



Lot 6wn: 



1 









.521 



2 



.557 

 .630 

 .597 





.557 

 .633 

 .547 



.573 



3 



.636 

 .504 



. 759 

 .749 



.669 



4 



.765 



5 



.630 



