32 BU.IJL,ETI]Sr 780, U. S. DEPARTMENT OF AGRICULTURE. 



Table IX. — Resistance of Nosema apis to drying at room, temperature. 



Date of inoculation. 



Results of inoculation. 



July 26, 1916. 

 Sept. 11, 1914 

 Aug. 11, 1916 

 Oct. 2, 1914.. 

 Sept. 1,1915. 

 Aug. 26, 1916 

 Oct. 16,1914. 

 May 24, 1916. 

 Sept. 1,1915.. 

 June 27, 1916. 



Nosema infection produced. 



Do. 



Do. 



Do. 



Do. 



Do, . 



Do. 

 No infection produced. 



Do. 



Do. 



From results recorded in Table IX it will be observed that Nosema 

 apis drying at room temperature remained virulent for from 56 to 

 60 days, that is, about 2 months. 



NOSEMA APIS DRYING AT OUTDOOR TEMPERATURE. 



Table X summarizes experiments in which Nosema apis was allowed 

 to dry at outdoor temperature. 



Table 'X.. — Resistance of Nosema apis to drying at outdoor temperature. 



Date of inoculation. 



Period of drying. 



Results of inoculation. 



Sept. 11, 1914 

 Oct. 2, 1914.. 

 Aug. ir, 1914, 

 Oct. 16, 1914. 

 June?, 1916.. 

 Sept. 27, 1913 

 July 9, 1915.. 

 June 27, 1916. 

 Aug. 17, 1915 , 

 July 17, 1916. 

 May 25, 1915. 

 July 17, 1914. 

 July 1,1914.. 



Months. 

 

 

 

 

 

 

 

 

 

 

 9 

 10 

 11 



Days. 

 21 

 42 

 46 

 56 

 60 

 60 

 75 

 80 

 85 

 100 





 10 







Nosema infection produced. 



Do. 



Do. 



Do. 



Do. 

 No infection produced. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



The results recorded in Table X show that Nosema apis ceased to 

 be virulent after 2 months of drying at outdoor temperature. 



NOSEMA APIS DRYING AT REFRIGERATOR TEMPERATURE. 



Table XI summarizes experiments in which Nosema apis was 

 allowed to remain dry in the refrigerator. 



Table XI. — Resistance of Nosema apis to drying at refrigerator temperature.^ 



Date of inoculation. 



Period of 

 drying. 



Results of inoculation. 



Dec. 2, 1915. 

 Jan. 3, 1916 . 

 Mar. 3, 1916. 

 Apr. 2, 1916. 

 Apr. 22, 1916 

 May 3, 1916. 

 Julys, 1916. 



Nosema infection produced. 



Do. 



Do. 



Do. 

 No infection produced. 



Do. 



Do. 



1 A few times during the experiments in which the refrigerator temperature was used, the ice became 

 exhausted, allowing the temperature to approach and possibly to reach that of the room. This higher 

 temperature, when present, however, at no time prevailed for more than a day. 



