NOSEMA-DISEASE. 41 



Table XXII. — Resistance of Nosema apis in dead bees kept at room temperature. 



Date of inoculation. 



Results of inoculation. 



Aug. 4, 1915. 

 Aug. 10, 1915 

 July 17, 1916. 

 Aug. 26, 1916 

 Aug. 17, 1916 

 Junes, 1916. 

 Aug. 23, 1915 

 July 26, 1916. 

 Aug. 1, 1916. 

 June 27, 1916 

 Aug. 20, 19U 

 Aug. 30, 1914 



Nosema infection produced. 



Do. 



Do. 



Do. 

 No infection produced. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



From Table XXII it is learned that when dead infected bees were 

 kept at room temperature the parasite remained virulent for three 

 or four weeks, but did not produce the disease after one month. 

 Since the temperature of the room was not constant, variations in 

 results obtained at this temperature are to be expected. 



AT OUTDOOR TEMPERATURE. 



Dead Nosema-inf ected bees were placed in a hive body standing in the 

 experimental apiary. At diflFerent intervals suspensions were made 

 and colonies were inoculated. In Table XXIII are summarized a 

 few experiments indicating by the results obtained the approxi- 

 mate period Nosema apis remains virulent in the body of dead bees 

 at outdoor temperature. 



Table XXIII. — Resistance of Nosema apis in dead bees drying at outdoor temperature. 



Date of inoculation. 



Results of inoculation. 



Oct. 19, 1914. 

 Aug. 23, 1915 

 Nov. 2, 1914. 

 June 7, 1916. 

 June 27, 1916 

 July 17, 1916. 



Nosema infection produced. 



Do. 



Do. 

 No infection produced. 



Do. 



Do. 



From Table XXIII it is seen that Nosema apis in the bodies of 

 dead infected bees kept dry at outdoor temperature remained viru- 

 lent for from five to six weeks. These experiments extended over a 

 period from June to November, as shown by the dates. It is to be 

 expected that if they had been conducted throughout the year the 

 results obtained would have shown a much wider range of variation. 



