22 BULLETIN 227, U. S. DEPARTMENT OF AGRICULTURE. 



Coal-tar creosote, Fraction II. 

 (PL II, figs. 12 and 13.) 



[Laboratory sample No. 1106. Cooperator. Semet-Solvay Co., Ensley, Ala. Naphthalene odor; nearly 

 solid at room temperature; specific gravity, 1.003 at 60~° C; flash point, 79° C; burning point, 85° C; 

 9 per cent distills below 205° C; 95.9 per cent distills below 287° C] 



Fungus. 



Tests. 



Times 

 killing 

 point 

 veri- 

 fied. 



Concentration of the preservative (per cent); 



Num- 

 ber. 



Dura- 

 tion. 



0.1 0.125 



0.15 



0.175 



0.2 



0.225 



Check. 



Fomes annosus 



20 

 18 



Weeks. 

 5 to 9 

 4 too 



\ ■•■--*• 



3 



4 



2 







1 



1 







4 

 4 

















Fomes annosus: In two weeks the radial growth of the check reached 20 mm.; no 

 growth on 0.15 per cent and above. In four weeks, no growth on 0.225 per cent. In 

 six weeks, 0.15 per cent reached 12 mm.; 0.2 per cent, 6 mm.; no growth on 0.225 

 per cent. The growth on the toxic media was compact and creamy in color. 



Fomes pinicola: In two weeks the radial growth of the check reached 40 mm.; no 

 growth on 0.125 per cent. In four weeks 0.1 and 0.125 per cent reached 40 mm.: no 

 growth above this point. The growth on the toxic media was a dingy white and less 

 fluffy than that on the check. 



Coal-tar creosote, Fraction III. 



(PI. Ill, fig. 30.) 



[Laboratory sample No. 1107. Cooperator. Semet-Solvay Co., Ensley, Ala. Liquid at room temperature; 

 specific gravity, 1.045 at 60° C; flash point, 103° C; burning point, 110° C; 0.9 per cent distills below 

 215° C. ; 4.7 per cent distills below 275° C. ; fraction from 1 70° to 245° C. moreor less solid with naphthalene.] 





Tests. 



Times 



killing 



point 



verified. 



Concentration of the preservative (per cent). 



Fungus. 



Num- 

 ber. 



Dura- 

 tion. 



o 

 d 



>2 

 o 



d 



d 



CM 



d 



d 



d 



d 



CO 



d 



"5 

 CM 



CO 



d 



O 



S 



o 





60 

 14 



Weeks. 

 4 to 12 













1 



1 



1 



1 







4 



F. pinicola 



4 to 7 



3 2 



1 









4 





















1 The radial growth of Fomes annosus on concentrations above 0.2 per cent is very slight, usually not 

 exceeding 1 mm. , and the actual toxic point is difficult to locate. It lies between 0.25 and 0.35 per cent. 



Fomes annosus: In 19 days the radial growth of the check reached 40 mm. ; 0.15 and 

 0.175 per cent, 1 or 2 mm. In five to six weeks 0.3 per cent showed 1 mm. and lower 

 concentrations 1 to 5 mm. The growth on the toxic media was creamy and slightly 

 lighter than that on the check. 



Fomes pinicola: In two weeks the radial growth of the check reached 40 mm. In 

 about one month 0.05 per cent showed 3 mm.; 0.075 per cent. 2 mm. In about seven 

 weeks 0.1 per cent showed initial growth; lower concentrations from 16 to 23 mm. 

 The growth on the toxic media was a fluffy white on the lower concentrations; denser 

 on 0.1 per cent. 



