THE TOXICITY TO FUNGI OF VARIOUS OILS AND SALTS. 



23 



Coal-tar credsote, Fraction IV. 

 (PI. II, figs. 14 to 19.) 



[Laboratory sample No. 1108. Cooperator, Semet-Solvay Co., Ensley, Ala. Liquid at room temperature; 

 specific gravity, 1.088 at 60° C; flash point, 130° C; burning point, 136° C; 0.9 per cent distills below 

 245° C; 54.3 per cent distills below 320°-C,] 





Tests. 



Times 



killing 



point 



verified. 



Concentration of the preservative (per cent). 



Fungus. 



Num- 

 ber. 



Dura- 

 tion. 



CN 

 O 



o 



o 



o 



o 

 o 



o 



o 



o 



o 





CN 



CO 



CO 



CO 



o 

 a) 



o 





75 

 23 



Weeks. 

 5 to 12 

 5 to 8 



1 

 1 













2 



2 



1 



1 



1 







4 





3 



2 



2 



1 







4 



















1 The growth of Forties annosus on concentrations above 2 per cent is very slight, usually not exceeding 

 1 nun. in six weeks, and the actual toxic point is difficult to locate. It lies between 2.5 and 3.5 per cent. 



Fomes annosus: In 12 days, the radial growth of the check reached 25 mm.; very 

 slight growth on 0.2 to 0.5 per cent. In 18 days, 0.8 to 3 per cent reached 1 to 4 mm. 

 In four weeks, 1.25 to 3 per cent reached 1 to 5 mm. The growth on the toxic media 

 was usually darker than on the check. 



Fomes pinicola: In two weeks, the radial growth of the check reached 40 mm.; 

 no growth on 0.025 per cent. In four weeks, 0.025 per cent showed 2 mm. ; no growth 

 on higher concentrations. In eight weeks, 0.025 per cent reached 25 mm.; 0.05 per 

 cent, 15 mm. ; 0.075 per cent, 10 mm. ; 0.1 per cent, 2 mm. ; no growth above this point. 

 The growth on the toxic cultures was dull, compact, and crinkled. 



Coal-tar creosote, Fraction V. 



(PI. II, figs. 21 to 24.) 



[Laboratory sample No. 1109. Cooperator, Semet-Solvay Co., Ensley, Ala. Heavy, tarry liquid; specific 

 gravity, 1.150 at 60° C; flash point, 172° C; burning point, 17S° C'.; 10.1 per cent distills below 320° C.J 

 63.3 per cent distills below 380° C] . 





Tests. 



Times 



killing 



point 



verified. 



Concentration of the preservative (per cent). 



Fungus. 



Num- 

 ber. 



Dura- 

 tion. 



0.1 



0.5 



1 



7 



7.8 



8 



30 



33 1 



Check. 



Fomes annosus 



42 

 30 



Weeks. 

 4 to 9 



4 to 8 





 



4 



3 



2 

 2 



2 

 1 







1 



1 







4 



4 

















1 Th?se values are based on gum-arabic emulsions. 



Fomes annosus: In 10 to 14 days, the radial growth of the check reached about 

 40 mm. ; 5.5 to 30 per cent showed 1 to 4 mm. In four weeks, 5.5 to 7.5 per cent reached 

 8 to 15 mm. ; 15 and 25 per cent, 2 mm. ; no growth on 20 per cent gum-arabic emulsion. 

 The growth on the toxic media was dense and creamy on the lower concentrations; 

 submerged at first on the higher concentrations. 



Fomes pinicola: In two weeks, the radial growth of the check reached 40 mm.; 

 in three weeks, 1 to 2 per cent showed initial growth; in four weeks, 3 to 6.5 per cent 

 showed first growth; after eight weeks, 4.5 to 5 per cent reached 10 to 20 mm. The 

 growth on the lower concentrations of the toxic media was white and fluffy; on the 

 higher concentrations it was tawny and radiate, with a crinkled edge. 



