EXPERIMENTS IN VACCINATION AGAINST ANTHRAX. 15 



opposite to where the vaccine had been injected. This animal was 

 given an injection of GO c. c. of serum and made a speedy recovery. 



In all, 399 animals, including horses, mules, cattle, sheep, and hogs 

 on farms where the disease had broken out, were treated with the 

 bureau serum and vaccine. Previous to this an aggregate of 10 

 cattle, 3 mules, and 13 hogs had died of anthrax on these farms. 

 On the morning of the day following vaccination a cow on one of the 

 farms died of anthrax. Exclusive of the above, no losses from 

 anthrax have occurred on any of these farms. 



Approximately 140 animals on several other infected farms were 

 vaccinated with a commercial vaccine by a representative of the 

 State live stock sanitary board. Within a day or two following 

 this vaccination it was reported 3 cows and 1 mule died of anthrax, 

 and since then 2 more cows have died of the disease. 



Another opportunity was afforded us to test the serum and vaccine 

 in an outbreak of anthrax in Noxubee County, Miss., where a num- 

 ber of farms were reported to be infected with the disease. A 

 quantity of serum and spore vaccine was furnished, and an inspector 

 detailed from the bureau station at Birmingham, Ala., to conduct 

 the work. On various farms where the disease had made its appear- 

 ance a total of 125 cattle were given the simultaneous treatment. In 

 addition 3 animals which showed symptoms of the disease were 

 given 30 c. c. of serum alone. No deaths from anthrax occurred 

 immediately following or since the vaccination, the affected animals 

 having all recovered from the disease: 



USE OF SERUM IN TREATMENT OF ANTHRAX IN MAN. 



Extensive data are available on the effectiveness of anthrax serum 

 for the treatment of the disease in man. It is recommended that 

 from 30 to 40 c. c. of serum be injected in three or four different 

 places. Should no improvement follow in 24 hours an additional 

 injection of 20 to 30 c. c. of serum should be administered. 



In most instances the results are very favorable, and this treat- 

 ment is acknowledged to be superior to any other mode of treatment 

 known for this disease. 



CONCENTRATION OF SERUM. 



Experiments are now being conducted in drying immune serum 

 with a view to preparing the same in pellet form. For this purpose 

 the serum has been dried in shallow pans in a serum-drying appa- 

 ratus. After thorough drying it is scraped from the pans, milled 

 into a fine powder, and prepared in a pellet machine into proper- 

 sized pellets. The spore vaccine is also being prepared in a similar 



