50 



BULLETIlSr 51, U. S. DEPARTMENT OF AGRICULTURE. 



Table 20. — Eggs with hJood rings — Continued. 

 SMALL SAMPLES OF EGGS WITH LARGE BLOOD RINGS. 



Sample 

 No. 



2 



o 

 m 



Date of 

 collec- 

 tion. 



Number of bacteiia 

 per gram at— 



-52 



o to 



O O (U 



p a c 



Percentage 

 of ammoni- 

 acal nitro- 

 gen, Folin 

 method. 



1 



1 

 o 



1 



o 



t.1 

 o 



Size of 

 sample. 



Remarks. 



d 



o 



d 



1 







4292 



Dl 

 D2 

 D2 

 D2 

 D4 



1912. 

 May 9 

 May 27 



...do 



May 28 

 July 9 



1,700 



100 



400 



100 



7,100,000 



400 

 100 

 750 

 150 

 400 





 



10 

 

 









3eggs 



12 eggs.... 



...do 



5 eggs 



8 eggs 



Broken yolks. 

 Do. 

 Do. 

 Do. 

 Do. 



440J 

 4-102 

 4430 

 4699 



0.0014 

 . 0016 

 .0019 

 .0018 



0. 0052 

 .0059 

 . 0070 

 .0065 



72.97 

 72.71 

 72.71 

 72.09 



LARGE SAMPLES OF EGGS WITH LARGE BLOOD. RINGS. 



4838 



4844 



48S4 



4S88 



41040 



F5 



July 25 



4,000,000 



1,900,000 



100,000 



0.0022 



0.0077 



71.41 



30 dozen.. 



F5 



July 26 



4, 300, 000 



3,100.000 



100 



. 0019 



. 0068 



72.17 



9 dozen . . . 



D5 



July 31 



Oin 1,000 



in 1 , 000 





.0019 



.0063 



70.00 



1\ pounds. 



D5 



Aug. 1 



6,500 



in 1 . 000 



10 



.0019 



. 0063 



69.96 



10 pounds. 



DG 



Aug. 22 



2,000,000 



1,400,000 



10,000 



.00:30 



.0071 



71.98 



7 pounds.- 



Kept in chill room 

 2 weeks. 



The results of the first four samples given in the second section of 

 Table 20 showed very few organisms and- no B. coli except in one 

 sample. The amomit of ammoniacal nitrogen was identical with that 

 found in contemporaneous samples of seconds, cracked, and dirtj'' 

 eggs, A fifth sample, taken in July, consisting of eight large blood 

 rings, gave a count of 7,100,000 bacteria per gram on agar plates 

 incubated at 20° C. and of 400 on similar plates kept at 37° C. The 

 divergence of the two counts is not explained. 



In Juh' and August larger lots of both small and large blood rings 

 were studied. These were caused in part by the warmth of the late 

 summer months and in part by a short period of incubation under 

 broody hens. The first observations were made of a case of blood 

 rings which had been held in a chill room at about 32° F. for two 

 weeks. The eggs Avere recandled and 8^ dozen eggs with broken yolks 

 or with 3"o]ks stuck to the shell were discarded. During the process of 

 breaking, the small and large blood rings w^ere separated, the basis 

 of division being less than 3 centimeters for the small and over 

 that for the large blood rings. ]\Iany of the eggs of the former type 

 had firm whites and yolks with faded rings. The eggs with the large 

 blood rings contained broken yolks. All eggs showing signs of mix- 

 ing of white and yolk, often termed " runny eggs," were excluded. 

 Each lot was mixed thoroughly by passing a few times through a 

 steamed sieve. The resulting mixtures had a good odor. The sam- 

 ple of small blood rings contained 3G,500 bacteria per gram; the one 



