MEDITERRANEAN FRUIT FLY IN HAWAII. 51 



tion, where the temperature ranged between 30° and 62° F., with a 

 mean of 46° F. for the period of observation, were still unhatched 

 after exposure for 14 days. After 14 days on Hualalai eggs were 

 carried in their host to Kealakekua and there hatched on February 

 27, or 17 to 18 days after they were deposited. 



Additional information on the duration of the egg stage has been 

 secured under cold-storage conditions. Eggs hatch in refrigeration 

 at temperatures ranging between 54° and 62° F. Five eggs depos- 

 ited August 15-16 and placed in a refrigerator at 54° to 57° F. on 

 August 16 hatched within the refrigerator on August 23, or in from 

 7 to 8 days after deposition; 19 eggs deposited at the same time 

 were removed unhatched on August 23 but hatched on the 24th, 

 outside the refrigerator, or 8 or 9 days after deposition. A single 

 egg also deposited on August 15-16 and similarly placed at»54° to 

 57° F. had not hatched by August 30, when it was removed to nor- 

 mal temperature, where it hatched within 24 hours, or 14 or 15 days 

 after deposition. Thirty-two eggs deposited on July 15-16 and 

 placed on July 16 at 58° to 62° F. hatched in the refrigerator on July 



20 or in from 4 to 5 days after being deposited, whereas 6 eggs of the 

 same lot were unhatched in storage on July 24, after which they 

 were removed to normal temperatures where they hatched on July 

 25, or 7 to 8 days after deposition. Eggs deposited on February 

 11-12, and placed at 48° to 52° F. on February 12, failed to hatch at 

 this temperature, but 20 removed after 14 days hatched within 16 

 to 17 days after deposition; 11 removed after 19 days hatched within 



21 to 22 days after deposition; while 1 removed after 22 days of 

 refrigeration hatched on March 8, or 24 to 25 days after deposition. 

 The examination of a second lot of fruit containing 1,853 eggs, de- 

 posited during a 24-hour period before they were placed at a tem- 

 perature of 48° to 53° F., showed that one egg hatched in storage 18 

 days after the inward date and after 24 and 27 days of refrigeration 

 64 and 56 first-instar larvae had hatched and died in the punctures. 

 Of these 1,853 eggs, 1,014 removed after refrigeration for 18 to 27 days 

 were dead; of 115 removed to normal temperatures after refrigera- 

 tion for 16 days, 35, 25, and 9 hatched in 17, 18, and 19 days, re- 

 spectively, after deposition. Eggs deposited during a 4-hour period 

 were placed immediately at 49° to 56° F.; after refrigeration for 21 

 days, 1 living and 7 recently dead first-instar larvae and 51 unhatched 

 eggs were found. Of other eggs deposited at the same time but 

 held at normal temperatures for from 44 to 47 hours before being 

 placed in storage, 8, 12, and 48 had hatched after refrigeration for 

 16, 19, and 21 days. One egg, two days after deposition, was held 

 in storage at a temperature of from 40° to 45° F. for 20 days (June 

 27 to July 17), when it was removed to normal temperatures where 

 it hatched three days later, or 25 days after deposition. One egg 



