LUPINES AS POISONOUS PLANTS. 25 



staggered when she attempted to walk. At 11.22 a. m. she stood 

 swaying from side to side. At 11.45 a. m. she was lying upon her 

 belly with her head on one side, her respiration being about 200. At 

 11.50 a. m. she was up on her feet and commenced to butt into the 

 fence again. She soon fell and lay panting. Her respiration was 

 160. At 11.57 a. m. she was lying upon one side and panting. At 

 12 noon she suddenly got upon her feet and started butting the 

 corners, but inmiediately fell over, holding her head on one side. 

 The chewing motions, as noted before, were marked. At 12.15 p. m. 

 she got upon her feet, tried to run but fell over; she picked herself 

 up, however, and started to butt into the corners. At 12.17 p. m. 

 she fell down and lay quietly. At 12.30 p. m. she was on her side 

 struggling in an attack of dyspnoea. At this time she was bled by 

 cutting the angular artery of the eye. At 12.35 p. m. her respiration 

 was 160. At 12.40 p. m. the movements of the jaws and lips still 

 continued. At 1.10 p. m. she was strugghng and the muscles of the 

 flanks were trembhng. Her pulse was so fast that it was impossible 

 to count it. Her temperature was 107.6° F. At 1.11 p. m. she had 

 fallen over on her side and was moving her body about. At 1 . 13 p. m. 

 her respiration was barely perceptible, and at 1.15 p. m. it stopped. 

 The movements of respiration and of the heart stopped at about the 

 same time. 



In the autopsy petechia were found upon the auricles of the heart. 

 The lungs were congested. The walls of the ileum were injected, with 

 some ecchymoses. The mesenteric blood vessels were injected. 



LABORATORY EXPERIMENTS WITH EXTRACTS OF LUPINE SEED UPON 



MICE. 



Preliminary experiments showed that mice are very susceptible to 

 poisoning by extracts of lupine seed injected intraperitoneally. 

 Accordingly, a few experiments were undertaken to determine by 

 this method the relative toxicity of extracts of various parts of the 

 lupine plant. Seeds, pods, and leaves of Lwpinus leucopsis collected 

 at GreycUff, Mont., in the summers of 1913 and 1914 were used for 

 these experiments. The following is a description of the method 

 used in preparing the extracts : 



Material wa.s taken from each part of the plant which it was desired to examine, 

 and all the samples were ground in the same mill set to the same degree of fineness. 

 They were all ground the same day and at approximately the same time. F'rom each 

 form of material a sample of 20 grams was taken, placed in a flask, and macerated 

 with 150 c. c. of Prolius's solution for 48 hours. The maceration of the different 

 samples examined was begun and ended at the same. time. Ninety c. c. of Prolius's 

 extract was then taken from each flask, filtered, and evaporated down spontaneously. 

 The re.sidue of each wa.s then extracted with 1 per cent of 1101, using 3 portions. 

 The solution wa-s filterf;d off, neiitralizc'd with NavOO;,, and made up to 10 c. c, so 

 that 1 c. c. of the solution was e'luivalent to 1.2 grams of the sample;. 



