26 



BULLETIN 405, U. S. DEPARTMENT OF AGEICULTUEE, 



These extracts were made by Mr. O. F. Black, chemical biologist 

 in the Office of Drug-Plant and Poisonous-Plant Investigations, and 

 were used in intraperitoneal injections upon mice. Table 2 gives 

 the results. 



Table 2. — Relative toxicity to mice of the various parts of-Lupinus leucopsis. 



Animal. 



Part of plant. 



Extract 

 used. 



Plant used. 





Mouse. 



Weight. 



Weight. 



Per 25 



grams of 

 animal. 



Result. 



No 27 



Grams. 

 24 

 26.5 

 23.5 

 14 



26.5 

 20 

 19 



15.5 

 19.8 

 19 

 24 

 19 



22.5 

 21 



Leaves 



C.c. 

 1 _ 

 . 75 

 . 5 

 .15 

 .5 

 .25 

 .15 

 .25 

 .3 

 .35 

 .2 

 .15 

 .2 

 .25 



Grams. 

 1.2 

 .9 

 .6 

 .18 

 .6 

 .3 

 .18 

 .3 

 .36 

 .42 

 .24 

 .18 

 .24 

 .3 



Grams. 

 1.25 

 .833 

 .64 

 .322 

 .565 

 . 375 

 .237 

 .482 

 .457 

 .552 

 .25 

 .237 

 .266 

 .357 





No. 35 



No. 44 



No. 47 



No. 36 



No. 37- . .. 



do 



do 



do 



Pods (seed removed).. 

 do 



Death in 9.5 minutes; 

 Death in 26 minutes. 

 Not sick. 

 Death in 4.5 minutes. 



No 45 



.do 





No. 38 



No. 43. 



Pods (seed shed) 



do 



Very sick; recovery. 



No 46 



do. 





No. 39 



No. 40. 



Seed (1914 collection).. 

 . ...do 



Death in 12.5 minutes. 

 Sick; recover}-. 

 Not sick. 

 Death in 14 Tiinutes. 



No. 41 



No. 42 



Seed (1913 collection).. 

 do 



Table 2 shows that the amount necessary to kill a mouse on the 

 basis of the standard weight of 25 grams is approximately as follows: 



Grams. 



Le3,ves 0. 6 to 0. 64 



Pods (seed removed) 3 to .35 



Pods (seed shed) 5 



Seeds, collection of 1913 3 to .35 



Seeds, collection of 1914 25 



Inasmuch as it is known from other experiments that the toxic and 

 lethal doses are practically the same, it is fair to assume that this 

 table gives, approximately, not only the lethal dose but also the 

 toxic dose. From Table 2 it appears that the seeds collected in 

 1914 were slightly more toxic than those collected in the preceding 

 year. Possibly a certain amount of the toxicity was lost in keeping. 

 It appears also that the pods from which the seed had been shed 

 were only about half as toxic as the seed. The pods from which the 

 seed had been removed were considerably more toxic than the pods 

 from which the seed had been shed, and the leaves were only about 

 one-half to one-third as toxic as the seed. These figures do not con- 

 form very closely to those obtained in the field experiments upon 

 sheep, but perhaps as closely as could be expected under the differ- 

 ent conditions of experimentation. An especially interesting feature 

 of this experiment was the definite proof obtained by Mr. Black by 

 analytical methods of the presence of the alkaloids in the leaves, 

 with the consequent deduction, confirmed by actual experiments, 



