10 



BULLETIX 408;, U. S. DEPAETMEE'T OF AGRICTJLTUEE. 



Table 6. — Destruction of fly larvss in horse manure — Results using 4 per cent solu- 

 tions of calcium cyanamid (series 1 and 2), and with dry calcium cyanamid and add 

 ])hosphate- mixtures (series 3 and 4)- 



[Open pile experiments, Baton Rouge, La., 1915.] 



No. 



22 



»3 



24 



Treatment of 8 bushels 

 manure. 



A, 10 gallons 4 per cent 



calcium cyanamid 

 solution 



B, Do 



C, Control 



D, Do 



A, 10 gallons 4 per cent 



calcium cyanamid 

 solution.." 



B, Do 



C, Control 



D, Do 



A, 3 pounds calcium cy- 



anamid4- 12 pounds 

 acid phosphate, 10 

 gallons water add- 

 ed 



B, Do 



C, Control 



D, Do 



A, 4 pounds calcium cy- 

 anamid +4 pounds 

 acid phosphate, 10 

 gallons water add- 

 ed 



Do 



C, 5 pounds calcium cy- 



anamid+5 pounds 

 acid phosphate ^ 



D, Do 



G, 3 pounds calcium cy- 

 anamid 4- 6 pounds 

 katnit^ 



H, Do 



E, Controls 



F, Do 



B, 



Flies 



Number. 

 0) 

 0) 

 0) 

 (') 



924 



1,865 

 9, 589 

 8,969 



70 



16 



263 



62 



4 



13 



511 



727 



Apparent 

 larvicidal 



action 

 tper cent 

 of control 



100 

 100 



90.5 

 79.9 



99 



Number 

 bacteria 

 per gram 

 manure 

 calculated 

 to dry 

 weight. 



MilHoTis. 



2,222 



600 



347 



857 



1,458 



1,154 



909 



723 



5,745 

 2,000 

 4,285 

 4,151 



182 

 545 



255 

 1,000 



1,220 

 511 

 431 

 511 



Ma- 

 nure, 

 total 

 nitro- 

 gen. 



Per ct. 

 0.735 



.653 



.688 



.428 

 .435 

 .449 

 .423 



.744 

 .646 

 .604 

 .540 



.625 

 .510 



.646 

 .510 



.681 

 .744 

 .414 

 .470 



Water extract. 



Alka- 

 linity, 

 cc N/20 

 NaOH 

 per 100 



ce (5 

 grams 



ma- 

 nure). 



7.35 

 6.85 

 7.00 

 7.50 



6.75 

 2.50 

 2.75 



3.75 



3.75 

 3.50 

 3.00 

 1.75 



3.75 

 5.50 



4.25 

 4.60 



6.25 

 6.85 

 3.10 

 3.35 



In per cent of total 

 nitrogen. 



Nitro- 

 gen. 



Per ct. 



45.03 

 35.26 

 30.93 

 34.30 



36. 68 

 27.59 

 21.16 

 29.31 



27.98 

 24.30 

 20.53 

 23.33 



26.88 

 35.30 



34.68 

 46.27 



36.27 

 45.29 

 27.05 

 27.44 



Am- 

 monia 

 nitro- 



Per ct. 

 6.26 

 7.28 

 6.74 

 5.96 



11.08 

 9.66 

 8.69 

 8.97 



8.07 

 7.89 

 5.79 

 7.04 



5.76 

 6.67 



6.81 

 9.02 



6.90 

 8.47 

 7.24 

 7.02 



Amid 

 nitro- 

 gen. 



Per ctr 



24.35 



18.22 



12.56 



8.28 



11.50 

 6.43 

 6.90 

 4.11 



10.73 

 13.78 

 3.48 

 3.33 



22.16 

 20.78 



21.37 

 23.92 



19.83 

 17.87 

 16.43 

 13.83 



1 Piles examined for larvae on third day after treatment. 



2 Nitrates were present in all samples of series 2, 3, and 4. 

 3 10 gallons of water added per 8 bushels of manure. 



Calcium Cyanamid and Kainit. 



The results given in Table 4, No. 1, where 1 pound of each 

 ingredient was used in duplicate tests, show varymg and incon- 

 sistent action, 50 and 81 per cent. Open-pile experiments given in 

 Table 6, No. 4, G and H, show that where 3 pounds of cyanamid 

 and 6 pounds of kainit were applied in mixture practically all the 

 larvse were killed. 



Chemical and bacteriological examuiations were made of samples 

 of manure taken from the last two mentioned experiments. The 

 bacterial coimt in one sample was twice as high as in the untreated 

 control samples and the other sample gave the same result as the 

 controls. The percentage of total nitrogen, as well as of the various 



