﻿EFFECTS ON HONEYBEES OP SPRAYING FRUIT TREES 



9 



Table 1. — Results obtained from chemical atmlyses of samples collected at 

 Winthrop, Me., 1914, after trees in full Moom had been sprayed 



Material analyzed 



Number of 

 samples anal- 

 yzed— 



Show- 

 ing 

 arsenic- 



Show- 

 ing no 



Aver- 

 age 

 weight 



num- 

 ber 1 



Aver- 

 age 



amount 

 of ar- 

 senic 



(As) per 

 indi- 

 vidual 



in sam- 

 ples 

 show- 

 ing 



arsenic 



Parts 

 of ar- 

 senic 

 (As) per 

 million 



Sprayed flowers, after removal of anthers and stigmas 



Anthers and stigmas of the flowers 



Pollen from legs of bees 



Fresh pollen stored in hives 



Honey, partially ripe. 



Nectaries from flowers sprayed in full bloom 



Nectaries from flowers sprayed after petals had fallen 



Dead bees from traps 



First wash, containing foreign matter from other bees from 



traps (no arsenic in subsequent washes) 



Nectar and pollen carriers 



Nectar and pollen carriers, after being washed five times 



Washings from 81 pollen carriers: 



First wash, containing foreign matter 



Second wash 



Third wash 



Fourth wash 



Fifth wash 



Nectar carriers, after removal of honey stomachs and i ntestines. . 



Honey stomachs of the nectar carriers 



Intestines of the nectar carriers 



Pollen carriers, after removal of honey stomachs andiatestines. 

 Pollen carriers, washed five times, then honey stomachs and 



intestines removed 



Dead bees from traps, after removal of contents of abdomens 



Contents of abdomens of the bees 



Dead bees from traps, washed five times, then contents of ab- 

 domens removed 



Dead pupffi from traps 



Dead larvae from traps 



Total number of samples analyzed 



Grams 

 4.24 

 .40 

 .43 

 1.18 

 7.40 

 Indi- 

 viduals 

 25 

 25 

 37 



95 

 30 

 30 



81 

 81 

 81 

 81 

 81 

 25 

 25 

 25 

 35 



100 

 34 

 31 



50 

 15 

 50 



Milli- 

 grams 



32 

 55 

 117 



0. 0003 

 .0007 

 .0004 



.0002 

 . 0005 

 .0003 



.0003 

 .0001 

 .0002 

 .0001 

 .0001 

 .0002 

 .0004 

 .0005 

 .0011 



.0001 

 .0003 

 .0002 



.0002 



1 Average weight per individual of sample, or average number of individuals per sample, taken for an- 

 auysis. 



A large niimbGr of dead bees from the traps bore on an average 

 0.0004 milligram of arsenic each, including arsenic both within and 

 without the body. To determine how much of this was carried out- 

 side the bodies, 95 other dead bees from the traps were washed four 

 times in a 20 per cent solution of arsenic-free concentrated nitric 

 acid and the fifth time in 05 per cent alcohol. These five liquids 

 (here called washes), containing the foreign matter from the bees, 

 were then analyzed; the first one contained 0.0002 milligram of 

 arsenic per bee, the other four none. 



Eight samples of live nectai' and pollen carriers, averaging 30 

 bees eacli, caught on the aligliting boards and killed in a cyanide 

 bottle, carried, externally and internally, 0.0005 milligram of arsenic 

 pei' ])i'Ai, or 25 per- cent more than w.is car-ried by the average of the 

 dead b(M;s from the trafjs. Foiirtcu^n other samples of live nectar 

 and poll<;n carriers, averaging 30 bcc^s each, caught and killed in the 

 same maimer, were washed five times, after which analysis showed 

 56707°— 26 2 



