620 On the Colours of Tealla crassicornis. 



The methods used were similar to those recorded in the 

 previous paper (1920). 



In the shore variety a very marked amount of haemoglobin 

 derivative (respiratory pigment) was found, but in the^deep- 

 water variety, even in red specimens, a much smaller quantity 

 Vi^as demonstrated ; in some cases only the less refrangible 

 band of the reduced alkaline solution was seen. Similarly, 

 the ether-alcohol soluble lipochromes were abundant in shore- 

 forms and scarce in deep-water forms. The positions of 

 their screens were but little different from those found in 

 Actinia, 



The above-mentioned lavender-tinted deep-water colour- 

 variety gave evidence of what seems to be new pigment, 

 which was extracted in an aqueous-alcohol layer underlying 

 the ether-alcohol extract. It was of a heliotrope-blue colour, 

 and found both in the tentacles and body-wall. This pig- 

 ment gave two well-marked bands, one in the yellow and 

 one in the blue. The band in the yellow almost corresponded 

 to the less refrangible band of oxy haemoglobin, but the band 

 in the blue was further to the right than that of the blood-pig- 

 ment (see figure). Treatment with either acid or alkali, even 

 when very dilute, caused the disappearance of the colour and 

 these bands. Ordinary pigment solvents — chloroform, ether, 

 benzol, etc. — did not take up this blue pigment, which does not 

 seem to agree with any previously recorded. Lankester has 

 described (1873) stentorine a blue pigment from the cortical 

 substance of a Stentoria which gave two strong absorption- 

 bands, one in the red and one in the green, similar to phyco- 

 cyan, the blue colouring-matter from Oscillarice. The action 

 of dilute acids (acetic, sulphuric, hydrochloric) did not appear 

 to effect any change, but the colour was intensified by the 

 addition of caustic potash, when the band in the red became 

 darker and the more refrangible band disappeared. 



M^Kendrick (1881) found that by macerating Cyanea in 

 sea-water a sky-blue fluid was obtained, showing two distinct 

 absorption-bands, one in the red and one in the orange. The 

 addition of ammonia produced a copious precipitate soluble 

 in acids, when the colouring-matter again goes into solution. 



Further, Blanchard (1882) extracted a blue pigment from 

 RMzostoma cuvieri in 90 per cent, alcohol, which gave three 

 bands, in the red, yellow, and green respectively — that in the 

 yellow being almost coincident with the D-line. 



Summary. 

 (1) The shore-forms o( Tealia are richer in both lipochrome 



