FILTRATION EXPERIMENTS ON THE VIRUS OF CATTLE 

 PLAGUE WITH CHAMBERLAND FILTERS "F".^ 



By E. H. RuEDiGEE. 



{From the Serum Section of the Biological Laboratory, Bureau of Science, 



Manila, P. I.) 



NicoUe and Adil-Bey ^ in the year 1902 reported that the artificial 

 peritoneal fluid (prepared by injecting a quantity of salt solution into 

 the peritoneal cavity of a bullock sick with cattle plague and collect- 

 ing the fluid about two hours later) frequently is infectious after it has 

 been passed through the Chamberland filter marked "F." 



Yersin ^ repeated the experiments of JSTicolle and Adil-Bey and 

 verified their results. 



In previous reports on this subject, I was not able to verify the results 

 obtained by the foregoing authors with the Chamberland filter "F." As 

 only a small number of filters had been iised, and those that were used 

 were chosen at random, I decided to repeat the experiments with selected 

 filters. 



I selected four of the coarsest from a large number of Chamberland 

 filters marked "F," the permeability of which I tested by passing distilled 

 water through them. I designated these as b, c, d, and e. Filter b 

 delivered a liter of distilled water in four minutes; filter c in five 

 minutes; filter d in six minutes, and filter e delivered a liter in eight 

 minutes. 



EXPEBIMENTAL. 



A quantity of artificial peritoneal fluid (for which I am indebted to 

 Dr. Thomson of the Bureau of Agriculture) was divided into five parts, 

 a, h, c, d, and e. Part a remained unfiltered ; part T) was passed through 

 filter b ; c through filter c ; d through filter d, and e was passed through 

 filter designated as e. 



Five bullocks were inoculated. 



Bullock No. 1 received 50 cubic centimeters of part a under the skin on the 

 6th day of January. On the ninth day of January the temperature rose rapidly 



* Read at the 6th Annual Meeting of the Philippine Islands Medical Associa- 

 tion, February 11, 1909. 



''Ann. Inst. Pasteur (1902), 16, 56. 

 ^ Ihid (1904), 18, 429. 



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