154 MARTINI. 



20, 21, and 24.) On tlic lOtli of February an attempt at culture of the Ijlnod 

 of this calf was again made, but contamination with bacteria occurred. 



On the 17th of February another attempt at cultivation of»the parasite was 

 made which resulted positively. On the 19th of February trypanosomata were 

 found in this culture. Diarrha?a began again on the 18th of February and led 

 to a rapid loss of strength and finally to the death of the calf on the 22d of 

 February. The death of the animal was probably due to stall infection through 

 carelessness of the stable boy, or to unfavorable conditions produced by artificial 

 feeding. 



Autopsy. — Marked emaciation, discharge from eyes and nostrils, lungs hyper- 

 aemic. The mesenteric glands were haemorrhagic and swollen to about the size of 

 a small bean. Catarrh of the large and small intestines was present. Otherwise 

 no pathological changes of importance were noted. 



The plasma bodies regarded by Koch as characteristic for Coast fever were 

 not found in smears from either the spleen or lymph glands. 



This experiment showed — 



1. The presence of an infection which belongs in the Texas fever 

 group. An infection with a variety of Coast fever did not enter into 

 the question, because the piroplasmata of this disease are not transferable 

 by a single blood inoculation, but only by repeated ones (Koch). 



2. The possibility of transferring the trypanosoma to animals by the 

 inoculation of cultures. The question of whether these trypanosomata 

 had developed from the piroplasmata remained imansweied; for the 

 culture succeeded only after the piroplasmata had been found in the 

 blood; on the other hand, the piroplasmata could be recognized in the 

 cultures up to the fifth day, during which time the trypanosomata had 

 shown a marked increase; stages of these parasites which might have been 

 regarded as transition forms from the piroplasmata were never seen. 



For fear that this animal (upon which ticks were discovered at the 

 beginning of the experiment) might have been infected with Texas 

 fever in spite of the precautions taken, it seemed necessary to repeat the 

 same experiment with another calf (see calf No. 3, below). 



Calf No. 2 {infected loilh fresh Mood). — February 5, 1909; new-born calf 

 from Indo-China, female, free from ticks; at first fed with mother's milk, later 

 after the death of the mother, fed artificially with preserved milk. Blood smear 

 free from piroplasmata and trypanosomata. Blood culture negative. A monkey 

 was given 30 cubic centimeters of its blood subcutaneously and remained healthy 

 and free from parasites. 



On the 5th of February the calf was injected subcutaneously with 30 cubic 

 centimeters of blood from the "original calf." 



On the 18th of February the first piroplasmata appeared in the blood and 

 were of the same appearance as those observed in the new-born calf No. 1. 

 (See also Plate II, fig. 1, I and d, and Plate V, figs. 23 and 25.) 



On the 19th of February the mother of the calf died with symptoms of 

 rinderpest. Artificial feeding was begun. On the 21st of February diarrhoea 

 began, leading to a rapid loss of strength and death of the calf on the 23d of 

 February. Cause of death probably the same as of calf No. 1. 



Autopsy. — Marked emaciation. Discharge from eyes and nostrils; mesenteric 

 glands haemorrhagic and swollen to the size of a pea or small bean. Catarrh of 



