PIROPLASMA AND TRYPANOSOMA OF CATTLE. 155 



the large and small intestines present. Liver shows slight icterus. Otherwise 

 no change noted. The plasma bodies of Koch were not found in smears from either 

 the spleen or lymph glands. 



The attempt to cultivate tiypauosoinata from . the blood of this calf 

 resulted negatively, although the culture medium showed no contamina- 

 tion with bacteria, which might have hindered the development of the 

 trypanosomata. 



The experiment with calf No. 3 showed — 



1. The existence of a variety of Texas fever as in calf No. 1 ; infection 

 with Coast fever was not considered for the reasons already mentioned in 

 the experiment with calf No. 1. 



3. The failure in this instance to transfer trypanosomata by means of 

 the fresh blood of the "original calf." 



Therefore, the results of the experiments on the first two calves showed 

 a striking contrast. Hence the question arose whether the piroplasmata, 

 which I had, to be su.re, not found on the fifth day of culture when I 

 injected the cultures of the blood of the "original calf" into calf No. 1 

 on the 5th of February, had nevertheless been present in the culture in a 

 condition able to cause infection, and whether they were not transferred 

 together with the trypanosomata and succeeded in developing in calf 

 No. 1. 



An experiment with another calf (No. 3) was planned to settle this 

 question. 



Calf No. 3 [repetition of the experiment on calf No. 1). — February 14, 1909; 

 new-born female calf from Indo-China; free from ticks; fed artificially. A daily 

 examination of the blood for parasites was made with negative results. A 

 culture from the blood remained sterile. A monkey was injected with 30 cubic 

 cantimeters of the blood and remained healthy and free from parasites. The calf 

 received subcutaneously on the 24th of February two seven-day-old cultures from 

 the blood of the "original calf." These cultures showed a good growth of 

 trypanosomata, but piroplasmata could no longer be found in them. At the 

 same time a monkey was given subcutaneously a similar seven-day-old culture. 

 The monkey remained free from parasites, but died on the 2d of April, 1909, 

 from an unknown cause. A blood specimen examined shortly after its death 

 revealed no parasites. 



The calf became sick on the 27th of February with diarrhoea, rapidly lost 

 strength, and died on the 3d of March. The cause of death was the same as in 

 calves Nos. 1 and 2. 



Autopsy. — Marked emaciation, catarrh of the large and small intestines; 

 mesenteric glands the size of a pea or a small bean. Otherwise no patiiological 

 changes were noted. The plasma bodies of Koch were not found in smear.s from 

 the spleen and lymph glands. 



A blood culture made on the 3d of March shortly before the death of 

 the calf developed a growth of trypanosomata, while piroplasmata were 

 never found in spite of repeated daily examination of the blood. 



This experiment resulted in the cultivation of trypanosomata in the 

 absence of piroplasmata. 



