158 MARTINI. 



On the 4tli of March tlie calf was injected subcutaneously with a 28-clay-old 

 culture containing trypanosomata which were feeblj' motile and in which piro- 

 plasmata were no longer found; other cultures were unfortunately not at hand. 



On the 13th of March an attempt at cultivation from the blood of the calf 

 was luisucce.ssful, although no contamination of the culture media with bacteria 

 resulted. 



On March 16 the attempt at cultivation was again unsuccessful, although there 

 was no contamination of the culture media with bacteria. 



March 17: Diarrhoea began Avhich led to a rapid loss of strength and death 

 of the calf on the 18th of March. The cause of death was probably the same 

 as in the cases of calves Nos. 1 to 4. 



Autopsy. — ^Marked emaciation; mesenteric glands the size of a pea to a bean; 

 catarrh of the large and small intestines. Otherwise no pathological changes 

 noted. The plasma bodies of Koch were not found in smears either from the 

 spleen or from the lymph glands. 



This experiment showed — 



The possibility of the failure of an attempt at infection of a calf with 

 the trypanosomata, from the injection either of too small an amount of 

 the culture, or of too old a culture, that is, of one which is weakened in 

 its virulence. The early death of the calf leads one to assume that this 

 conclusion is only conditionally correct, since, if the animal had lived 

 longer, further attempts at culture might have shown finally, the presence 

 of an infection with trypanosomata. Nevertheless the experiment was 

 valuable as indicating in future experiments the inadvisability of 

 employing for inoculation the contents of a single culture tube or that 

 of one so old. 



EXPERIMENTS AVITH CALVES EEOM INDO-CHHSTA AND AUSTRALIA. 



The experiments with the following calves were made in a new stable 

 protected against rinderpest and other infection that could be acquired by 

 contact. The calves were well beyond the first days of life. No. 6 was a 

 calf from Indo-China not quite a month old; Nos. 7, 8, and 9 were 

 Australian calves four to five months old. They were all free from 

 ticks. Calves Nos. G, 7, and 8 were fed with preserved milk and dry food, 

 calf No. 9 with dry food alone. 



Calf No. 6 (repetition of the experiments with calves Nos. 2 and 4)- — March 

 26, 1909; a male calf from Indo-China; free from ticks; blood smears free from 

 parasites ; blood culture negative. Smears made from the surface of the cen- 

 trifugated, defibrinated blood were free from parasites. Thirty cubic centimeters 

 of its blood were injected subcutaneously into a monkey; the monkey remained 

 healthy and free from parasites. 



March 26: The calf received subcutaneously 50 cubic centimeters of the blood 

 of the "original calf." 



April 3: Piroplasmata found in blood smears. 



On April 3, 6, 10, and 14 and May 4 attempts at blood cultures were negative, 

 as regarded trypanosomata, although there was no contamination with bacteria. 



Piroplasmata like those seen in the other calves were found in the 

 cultures until the 24th of April. They were usually scarce. A change 



