PIROPLASMA AND TRYPANOSOMA OP CATTLE. 169 



Plate V. 



Fig. 19. Culture of trypanosomata photographed alive in a hanging drop prepara- 

 tion; from a bouillon culture of the "original calf" made on January 

 18, 1909, photographed on January 23, 1909. Magnification about 

 450 diameters. 



Fig. 20. Form like Pirosoma bigeminum in the fresh blood of calf No. 1. (Free 

 form. ) 



Fig. 21. Intracellular forms observed on the same day as the forms illustrated in 

 fig. 20. 



Fig. 22. Intracellular forms observed on February 20, 1909, prepai-ation from the 

 fresh blood of calf No. 1. 



Fig. 23. A form resembling Pirosoma bigeminum from the fresh blood of calf No. 

 2. (Free form.) 



Fig. 24. Intracellular form resembling a trypanosoma found in the fresh blood of 

 calf No. 1. 



Fig. 25. Intracellular form resembling a trypanosoma found in the fresh blood of 

 calf No. 2. 



Fig. 26. Involution forms of trypanosomata found on the fifth day of a culture 

 made from the blood of the "original calf." 



Fig. 27. Trypanosoma evansi from the calf found infected with surra. 



Plate VI. 



Fig. 28. A group of the developmental forms of Koch ; Pirosoma bigeminum on 

 the second day of cultivation in bouillon; parasites lying between 

 the red blood cells. Preparation from calf No. 7. 



Fig. 29. Larger group of the same forms observed the same day as those 

 illustrated in fig. 28. Parasites lying between red blood cells and 

 leucocytes. Preparation from blood of calf No. 7. 



Fig. 30. Ray form of Koch. Preparation from blood of calf No. 4. 



Figs. 31 and 32. Form with chromatin points described also by Koch. Preparation 

 made from blood of calf No. 4 on the third day of cultivation. 



Fig. 33. Large oval form with three chromatin masses and four chromatin tips, 

 together with small developmental forms lying between the swollen 

 erythrocytes. Preparation made from calf No. 4 on the third day of 

 cultivation. 



Fig. 34. Large oval form, lying isolated between swollen erythrocytes. Prepara- 

 tion from calf No. 4 on the third day of cultivation. 



