336 



RUEDIGER. 



Table TI. — Antitetanic serum diluted mth physiological salt solution in propor- 

 tion of l-J/, unfiltered and filtered tested on guinea pigs. 



Quantity of — 



Portion of serum. 



Diluted 

 serum. 



Toxin. 



cc. 



ijii 



W 



Lethal 

 donen. 



100 



100 



100 



100 



100 



100 



100 



100 



100 



Re.sults. 



Lived. 



Lived. 



Died. 



Died. 



Lived. 



Lived. 



Died. 



Died. 



Lived. 



5 (\ () 



100 



Died. 



nhr 



100 



Lived. 



-M 



100 



Died. 



■tha 



100 



Lived. 



^U 



100 



Lived. 



2^*1 



100 



-Died. 



2Bff 



100 



Died. 



The results obtained sliown in Table II show the neutralizing value 

 of the filtered serum to be practically identical with that of the unfiltered 

 serum. 



Bacteriological examination showed portions N and W free from 

 bacteria. 



EXPERIMENT NO. III. 



A quantity of antidiptheritie serum was divided into fovir portions A, V, N 

 and W. Portion A was not filtered while portions V, N and W were passed 

 through Berkefeld filters V, N and W respectively. 



Varying quantities of these different portions were mixed with a test 

 dose of diphtheria toxin, allowed to stand for thirty minutes and were 

 injected under the skin of guinea pigs weighing between 245 and 355 

 grams. 



