REACTION OP CULTURE MEDIA. 



343 



Table 1. — The change in morphology of cholera vibrios — Continued. 



— = clear. 



± — a trace of turbidity. 



slight turbidity, 

 moderate turbidity. 



+ -|- H- = marked turbidity. 

 + -!- + + = maximum turbidity. 



JNo. 



Reaction of peptone 



Strain ''Valentine." ■ 



solution. 



Turbidity. 



Morphology. 



1 

 2 

 3 



4 

 5 



! 6 



\ 7 

 8 

 9 



Acid, 1.5 per cent 



Acid, 3.0 per cent 



Acid. 0.5 per cent 



Neutral 



Alkaline, 0.5 per cent 



Alkaline, 1.0 per cent 



.Alkaline, 1.5 per cent 



Alkaline, 2.0 per cent 



Alkaline, 2.5 per cent 



\ j 

 ± ? 1 Not visible in stained preparations. 

 ± ? j Not visible in stained preparations.. 

 + j Small, straight, slender rods. 

 4 4-+ ! For the most part curved, some straight. 

 ^ i + Typical vibrios, somewhat .short and thick. 

 -;-i +4 Thick and short organisms, approaching the oval 

 form, 

 4- 1 For the most part oval forms, a few curved. 

 ± ? j Very short bacilli and some spherical forms. 



± ? I A few coccus forms. 



j 



v„ Reaction of peptone 



1 

 Strain "Mendoza." 



i 



.solution. 



Turbidity. ' Morphology. j 



1 

 2 



-' 

 4 



5 



6 



7 

 8 

 9 



Acid, 1.5 per cent 



Acid, 1.0 per cent 



Acid, 0.5 per cent 



Neutral 



Alkaline, 0.5 per cent 



Alkaline, 1.0 per cent 



Alkaline, 1.5 per cent 



Alkaline, 2.0 per cent 



Alkaline, 2.5 per cent 



±? 



±? 



± 

 + + 4- 

 + + + + 

 + + 4- - 



Not visible in stained preparations. 



Small oval bacilli. 



Organisms longer than in No. 2. 



Curved forms appear. 



For the most part curved; some rods. 



Organisms all typical and thick. 



Very thick and short, some have the oval form. 



All organisms have the oval form. 



Spherical forms. 



Five 3-ears ago KoUe and Gofschlich published a report on the bac- 

 teriological diagnosis of Koch's cholera vibrio. They collected 65 

 strains of the cholera organism and were able to prove that all of these 

 strains conld be identified by the reactions of agglutination and bac- 

 teriolysis with a cholera immune serum. Although these strains did 

 not differ in their biological characteristics, yet they did show mor- 

 phological differences. Table 8 indicates the morphology of the strains 

 studied bv KoUe and Gotschlich.* 



* Ztschr. f. Hyg. (1903), 44, I. 



