346 OHNO. 



Table 3. — Showing the development of cholera vibrios from suspected cholera stools 

 in peptone solutions of different reaction. 





Num- 

 ber of 

 strain. 



Kcnetion.s. 



Chol- 

 era? 



Num- 

 ber of 

 .strain. 



Reactjon.s, 





Acid 

 0.3 per 

 cent. 



Alka- 

 line 0.5 

 per 

 cent. 



Alka- 

 line 0.5 

 per 

 cent. 



Acid 

 0.3 per 

 cent. 



Alka- 

 line 0.5 

 per 

 cent. 



Alka- 

 line 1.5 

 per 

 cent. 



Chol- 

 era? 



Development. - 



Form 



! »: 



i: 



) 



~ 



1 " 



IT 

 1- 



-l-± 

 ) 



) 



+ ± 

 ) 





1 ^ + 



43 \l - 



1 



44 i ) 



45 li ) 



) 



-f-;- + 



9 



-r- + 

 ) 



^- 

 ) 



'V\. 



Indol 



1 



Development.. 



Form 



Indol 



Development-- 



Form 



Indol 



Development- 

 Form . 



I 37 

 i 38 



1 39 



1 



1" 



) 

 -1- 



) 



H- 



-1- + 

 ) 



■ + 



Indol — 



+ . +± 1 



- -1- 



- ) 1 



Development.. 



46 



IT 



-h-i- 

 ) 



+-t- 

 ) 



•> 



-f 



7 



-1- 



1^ 



■ + 1 



Indol . - 



± 

 -\-^- j + + 

 — 1 1 



1 

 1- 



Development- 

 Form 



f + + + 

 47 { ? 

 1 - 



Indol- - 



+ 



















) Indicates the presence of comma-shaped vibrios. 



It is very easy to make a bacteriological diagnosis from typical cholera 

 stools, and, after the organisms liave been obtained in pure culture, 

 it is not difficult to differentiate and identify them by means of 

 agglutinative and bacteriolytic reactions ; however, it is nevertheless very 

 difficult to isolate the cholera vibrio when it is present in very small 

 numbers in the suspected material, the reaction of which is unknown 

 and in which the organism sometimes appears with a different mor- 

 pholog}^ 



If, in the beginning of a bacteriological examination, microscopical 

 preparations are made from the fseces directly or from the peptone 

 used for enriching, we may fail sometimes entirely to recognize the 

 presence of the cholera vibrio in such preparations because of the 

 divergent morphology which it may assume, and therefore neglect 

 further to pursue the bacteriological examination. Therefore during 

 a cholera epidemic where it is necessary to examine bacteriologically 

 a very large number of specimens of fseces for diagnosis, my proposal 

 to use culture media of three different degrees of alkalinity or acidity 

 so that the different strains of the cholera vibrio will appear in typical 

 vibrio or comma form in at least one of them, notwithstanding the 



