352 



OHNO. 



Table 8. — Relation between the reaction of the peptone solution and the production 



of indol. 



Series 



of 

 tubes. 



Hoft'man. 



Felix II. 



Helmon I. 



Juan I. 



Reaction of tubes. 



Devel- 

 opment. 



Indol. 



Devel- 

 opment. 



Indol. 



Devel- 

 opment. 



Indol. 



?v^^^-^o^. 



1 

 2 

 3 

 4 

 5 

 6 

 7 

 8 

 9 



"I" 



=k? 



+ ) 



n-4-H- 

 -H + 



-1- 



± 

 +? 



i 



-) 



+ + 



+ + 



-f + -l- 

 + + -i- 

 + -I- 



+ 



4 



-) 

 -) 

 + +) 

 -) 

 - ) 



1 



+± 



+H- 



-f-t- 

 ± 



T 



+ ) 

 + ) 



Acid, 1.5 per cent. 

 Acid, 1.0 per cent. 

 Acid, 0.5 per cent. 

 Neutral. 



Alkaline, 0.5 per cent. 

 Alkaline, l.Opercent. 

 Alkaline, 1.5 per cent. 

 Alkaline, 2. Oper cent. 

 Alkaline, 2.5 per cent. 



) Indicates typical jnorphology of the cholera vibrio. 



Table 9. — Examination of stools from individuals who had been in contact with 

 cholera patients (no cholera vibrios present) ; showing the development of other 

 vibrios, than the cholera vibrio, when cultivated in the 3 peptone solutions of 

 different reaction and their relation to the production of indol. 





Num- 

 ber of 

 strain. 



Reaction. 



Chol- 

 era? 



Num- 

 ber of 

 strain. 



Reaction. 





Acid, 

 0. 3 per- 

 cent. 



Alka- 

 line, 

 0. 5 per- 

 cent. 



Alka- 

 line, 

 1. 5 per- 

 cent. 



Acid, 

 0. 3 per- 

 cent. 



Alka- 

 line, 

 0. 5 per- 

 cent. 



Alka- 

 line, 

 1. 5 per- 

 cent. 



caoi- 

 era? 



Development. - 

 Form of vibrio - 



Indol 



Development.. 

 Form of vibrio. 

 Indol 1 



i (iO 



r "^ 

 62 



I fi3 



1- 



Ii5 



1 — - ± 



1 : ' ■ : 



1- 



1 



1- 



1- 



1 



H7' 

 6S' 

 69< 



-!• + + 



+ 



+ 4 



) 



). 



4- + H- 

 ) 



-' + + 

 1 



H- '--- 



4-4- + 



4-4- 

 ) 



4-4- 



) 



__ 



4--!- 

 ) 



4 

 ) 



4-4- 

 ) 



4-± 



± 



4-4- f 

 ) 



4 



■f-h 



) 



) 



+ 



} ~ 



1- 



Development.- 

 Form of vibriO- 



Indol 



development— 

 Form of vibrio. 



Indol 



Development.. 

 Form of vibrio. 



Indol 



Development. - 

 Form of vibrio. 

 Indol 



F 



[ ) 



-f-f- 



4-4- 

 ) 



+ •■ 

 ) 



i- 



+ + + 



) 



As to the relation between the cholera vibrio in culture media of 

 different reaction and its agglutinabilit}', it would appear from the 

 results of my experiments that the more the acidity or alkalinity of 

 the media is increased the more the agglutinability of the organism is 

 decreased. Further observations on this question are now being pursued. 



