408 CLEGG. 



givfci-iiiL'-iigar, LoclHcr's blood yei'uni, lactose-agar, Dorsctt's egg inediuiu, 

 glufose-bouilion and giyofrine-bouillon. The optimiuii teinperature for 

 growth was 3?° C; growtli will take place at a lower teiiiperatiu'e, but 

 more slowly. 



)<tirfacc colonies on nutrient agar. — These colonies when the}' first 

 become visible are raised and possess smooth edges. They measure 

 about 1 millimeter in diameter, are compact in appearance and. imdei' 

 the low power of the microscope, show a brownish pigment which is 

 ]nore jjlentiful in the center of the colony than at the periphery. 



Nutrient agar drole. — A slightly raised, moist growth appears after 

 three days, producing a brownish-yellow pigment. 



Plain agar strol-e. — Growth appears after three days as slightly raised, 

 discrete colonies, which later become confluent and moist, producing a 

 bright orange pigment. 



Potato. — After three days a raised, moist, creamy growth appears, pro- 

 ducing a bright orange pigment. No change in the medium occurs. 



Alkaline litmus-milh. — Growth occurs on the surface of the medium 

 as a yellow membrane. The milk gradually loses its color, hut does not 

 become acid. A sediment is deposited at the bottom of the tube. 



Glucose-agar strohe. — An abundant growth appears on the third day 

 as a raised smooth moist surface with a bright orange pigment. The 

 growth can be lifted in heaps on a platinum loop and may be readily 

 suspended in an aqueous solution. 



Glycerine-agar sirol-c. — Growth is similar to that on glucose agar. 

 Jt may develop from a single stroke to such an extent that the entire 

 surface of the medium is covered, giving the appearance of a heaped-up 

 creamy substance. The growth is not adherent to the medium. 



Loeffler's Mood scrum. — Growth is slower on this medium. It is 

 slightly raised and moist, and very little pigment is produced. Lique- 

 faction begins in ten days and is complete in twenty days. 



Glucose stab. — Very little growth occurs along the needle tract. It is 

 ])j'ol'use on the surface of the mediun). 



Lactose stah. — Growth is similar to that in glucose stab. No change 

 in the reaction of the medium occurs. 



Dorsett's egg medium. — Growth is profuse on this medium after three 

 days. The appearance is similar to that on glycerine agar. 



Glucose-bouillon. — Growth appears as small patches on the surface of 

 the medium. These later coalesce and form a wrinkled membrane. A 

 sediment gradually forms at the bottom of the tube. The medium 

 ]-emains clear. 



Glycerine-bouillon. — Gi-owth is similar to that in glucose bouillon. 



"Fermentation tubes showed no production of gas, nor were acids 

 formed in dextrose and lactose bouillon. 



Resistance to lienl. — Suspensions of the cultures in salt solution were 



