410 CLEGG. 



method of staiuing the central portions of the bacilli retain the staiii to 

 a greater degree than do the poles. 



A resume of the successful cultures including those rejjovted in the 

 preliminary paper follows : 



Bacillus A. — An acid-fast bacillus cultivated from the spleen of a 

 leper and growing in symbiosis with Amoeba dysenteria' and several 

 other varieties of bacteria. 



Bacillus B. — An acid-fast bacillus cultivated from the spleen of a 

 leper and growing in symbiosis with Amoiba dysenteria and a number 

 of unidentified bacteria. 



Bacillus G. — An acid-fast bacillus cultivated from the nodules in the 

 ears of a leprous patient and growing in symbiosis with amoebae and a 

 number of unidentified bacteria. 



Bacillus D. — An acid-fast bacillus cultivated from the nodules in 

 the ears of a leprous patient and growing in symbiosis with Ammba 

 dysenteria} and a number of unidentified bacteria. 



Bacillus E.- — An acid-fast bacillus cultivated from the nodules in 

 the ears of leprous patients and growing in sympiosis with Ama'ba 

 dysenterice and a mixed variety of bacteria. 



Bacillus F. — An acid-fast bacillus cultivated from the spleen of a 

 leper and growing in sjrmbiosis with an amoeba isolated from water, and 

 with the cholera vibrio, obtained in pure culture and transplanted re- 

 peatedly on various culture media. 



Bacillus 0. — An acid-fast bacillus cultivated from a nodule in the 

 thigh of a leprous subject and growing in symbiosis with a water amoeba 

 and cholera vibrio and in pure culture. 



ANIMAL EXPERIMENTS. 



Monkey No. 4440 {Gynomulgus philippinensis Geoff.) was inoculated 

 subcutaneously with a suspension of four loops of a ten-day culture of 

 Bacillus A, Ammba dysenterice and a variety of unidentified bacteria. 



The animal died three months later and the autopsy showed no evi- 

 dence of leprosy or of tubercular lesions. 



Guinea pig No. 4484 was inoculated in the peritoneal cavity with one 

 loop of a ten-day growth of Bacillus A, Ama'ba dyscnteriw and symbiotic 

 bacteria. The animal died five weeks later from lobar pneumonia. The 

 autopsy showed no evidence of lepros}-, or tuberculosis. 



Guinea pig No. 4485 was inoculated subcutaneously with a suspen- 

 sion of one loop of a ten-day culture of Bacillus A, Amaiba dysenterice 

 and symbiotic bacteria. Five weeks later it was found in a dying con- 

 dition and was chloroformed. Autopsy showed haemorrhagic areas located 

 on the extremities, chest and abdomen. There was markedly cedematous 

 tissue surrounding the joints. The heart was covered with small pete- 

 chial hemorrhages. All the vessels were injected. The lungs were 



