CULTIVATION OF LEPROSY BACILLUS. 411 



noraial in consistency and no tubercles were present. All the glands 

 were swollen and some, especially the mesenteric and inguinal, were 

 liEemorrhagic. 



Cultures made from the organs were negative. 



Guinea pig ISFo. 4486 was inoculated in the peritoneal cavity with a 

 suspension of one loop of acid-fast Bacillus E, Amoeba dysenterioe and 

 symbiotic bacteria. The animal died five weeks later. The autopsy 

 findings were similar to those described in guinea pig No. 4485. 



Guinea pig No. 4487 was inoculated in the same manner as the pre- 

 vious animal. The animal died six weeks later with similar lesions. 

 There was no evidence of tuberculosis. Cultui'es made from all the 

 organs remained sterile. 



Guinea pig No. 4488 was inoculated in the peritoneal cavity with a 

 suspension of one loop of a ten-day culture of Bacillus P in symbiosis 

 with the cholera vibrio. The animal died one month later. There 

 were severe hsemorrhages in all the organs. There was no evidence of 

 tuberculosis. 



Guinea pig No. 4483 was inoculated subcutaneously with one loop 

 of a ten-day culture of Bacillus F and cholera vibrio. The animal died 

 five weeks later with similar lesions. There was no evidence of tuber- 

 culosis. Cultures made from all the organs remained sterile. 



The foregoing animal inoculations, while giving us little information 

 in themselves as to the pathogenicity of the acid-fast organism, serve to 

 separate the latter from the tubercle bacillus. In none of the injected 

 animals were lesions found that resembled those of tuberculosis. The 

 following animal experiments were made witli one of the pure cultures 

 of the acid-fast bacillus: 



Guinea pig No. 4596 was inoculated subcutaneously with one loop 

 of a ten-day pure culture of Bacillus F, suspended in 1 cubic centimeter 

 of salt solution. After one week, an indurated nodular thickening ap- 

 peared at the site of inoculation. This thickening and induration 

 gradually spread and in four weeks the whole abdominal surface was 

 involved. The hair of the skin surface corresponding with the lesion 

 just described fell out, and before the exitus the animal became greatly 

 emaciated; it died four weeks after the inoculation. Autopsy showed 

 a mai'ked thickening of the subcutaneous tissues of the abdominal 

 wall, in some parts measuring 3 millimeters in thickness. Tliere 

 was hsemorrhagic infiltration of the muscular tissue as avcII as ai'oimd 

 the joints of the extremities. The abdominal cavity contained no fluid. 

 The heart was slightly dilated. The lungs appeared normal. The 

 spleen was enlarged to twice its normal size and was firm in consistency. 

 Cut section showed an increase of fibrous connective tissue. The liver 

 M-as enlarged and congested, presenting a dark, nearly black color. The 

 kidneys showed s.ome cloudy swelling. The stomach and intestines ap- 



