412 CLEGG. 



peared normal, ("ultures made from the subcutaneous lesions were 

 negative. Smears made from the lesions and stained by the acid-fast 

 method showed the presence of large numbers of acid-fast hacilli, the 

 majority of which were intracellular. 



A portion of the nodule was phiced in Zcnkei*'s fluid, hardened in the 

 usual manner in alcohol and embedded in paraffine. The cut sections 

 cut at four microns thickness were stained by a modified Borrel metliod, 

 and also with, eosin, followed by Unna's alkaline methylene blue. 



The section includes muscular and subcutaneous tissue and nerve 

 bundles. The nerves, and for the greater part the muscle fibei-, show 

 no abnormalities. The usual fibrous subcutaneous tissue has been re- 

 placed by richly cellular granulation tissue, consisting almost exclusively 

 of irregularly spindle-shaped, young connective tissue cells. Near the 

 cutaneous border of the section is found an oval-shaped, well-defined 

 area consisting chiefly of leucocytes and necrotic tissue debris. In the 

 rest of the section, leucocytes are present in very small numbers. In 

 many of the cells of this tissue are found numerous short bacilli, some 

 lying singly, but usually in dense masses in the protoplasm. These 

 bacilli are stained deeply with the magenta in the Borrel sections and 

 with methylene blue in the eosin-methylene blue preparations. In a 

 few places the granulation tissue has involved the muscle bundles, and in 

 this situation cells containing the bacteria are seen. Cells containing 

 two or three distinct nuclei ai'e found and in a few sections t\'pieal giant 

 cells are seen with marginal nuclei. The blood vessels in the section 

 contain a relatively large number of leucocytes. 



The section when stained with carbol-fuchsin showed the presence of 

 acid-fast bacilli corresponding in number witli those found in the section 

 just described. 



Guinea-pigs Nos. 4597 to 4598 and 4599 were inoculated in a 

 similar manner to Guinea-pig JSTo. 4596. All died in tlie fourth week 

 with the same symptoms and similar anatomical changes. 



Cultures were made from the organs of these animals on media con- 

 taining amcebse and the cholera vibrio and on various laboratory media. 

 In one instance only was the acid-fast microorganism reclaimefl. The 

 growth in this ease occurred on tlie media containing the amoeba and the 

 cholera vibrio. 



Guinea-pigs iSTos. 4600, 4601, 4602 and 4603 were inoculated in- 

 traperitoneal! y with one loop of a ten-day cultiire of Bacillus F sus- 

 pended in 1 cubic centimeter of salt solution. All the animals died 

 during the fourth week. The autopsies sliowed marked haemorrhages in 

 the deep muscular tissues. The spleen was greatly enlarged in all the 

 animals. There M'as severe congestion of the liver. There were no 

 tubercles present and no cutaneous lesions. Cultures made from the 



