4 1 4 CLEGd. 



cultivated i'rom a lesion in the guinea pig. Tlxese guinea pigs, as well 

 as others which received intraperitoneal injections, invarialjly elied during 

 the third or fourth week after inoculation. 



Inoculations into monkeys have yielded as yet only negative results. 



It seems strange, at first thought, that, although tlie fii'st culture of 

 the leprosy bacillus on artificial media is attended with so much difficulty, 

 it should grow so readily in subsequent transplants; but the tubei'cle 

 bacillus often acts in a strikingly similar manner, only one or two tubes 

 out of twenty or thii'ty yielding a growth from tuberculous tissue, 

 whereas subcultures from this growth all develop luxuriantly on various 

 uK'dia. 



The majority of the lepei'S from whom the material for these cultures 

 was obtained, had tubei-eulous lesions in their lungs, Ijut the tissues 

 used for the cultures showed no evidence of tuberculosis. The animal 

 inoculations described in the text prove that the acid-fast organism 

 cultivated Ijy me is not the tubercle bacillus. 



SUJIMARY. 



1. The leprosy bacillus was first cultivated from leprosy material in 

 symbiosis with other unidentified bacteria and amoebae and later from 

 other cases in s)-mbiosis with amoeb?e and the cholera vibrio. 



2. By heating a symbiotic culture of amoebae, cholera and leprosy for 

 a half hour at 60° C. and incubating, the leprosy bacillus was obtained 

 in pure culture. 



3. The leprosy bacillus isolated in this manner grows readily on the 

 ordinary lal)oratoi'y culture media. 



4. The bacillus is pathogenic for guinea pigs, subcutaneous inocula- 

 tions having caused lesions which macroscopically and microscopically 

 resemble the leprous lesion^ of human subjects. 



