40 



CHAMBERLAIN, VEDDER, AND WILLIAMS. 



was prepared according to the method previously described (i), 

 except that the poHshings were extracted by three successive 

 macerations with fresh 95 per cent alcohol and that the residue 

 obtained by this extraction of 10 kilograms of polishings was 

 redissolved in 1 liter of distilled water instead of the usual 

 10 liters. These two changes of method were made in order 

 to obtain a more concentrated extract which would be better 

 suited for chemical analysis. The extract used in the following 

 experiments, therefore, is several times stronger than that pre- 

 viously used. The results of its analysis are shown in Table II. 



Table II. — Analysis of a neuritis-preventing extract of rice polishings, 

 viade more concentrated than that shown in Table I. 



Constituents. 



Specific gravity- 

 Total solids 

 Sucrose ^ 

 Reducing sugars 

 Total nitrogen 

 Amido-nitrogen 

 Phosphorus pentoxide 

 Ash 

 Undetermined 



Per cent. 



1.0437 

 12.517 

 6.33 

 0.52 

 0.161 

 0.156 

 0.006 

 0.27 

 5.23 



" The determination of sucrose was not accurate because of the presence of other optically 

 active substances. 



Nonproteid nitrogenous substances are commonly found in seeds and in 

 other parts of plants. Thus, in Lupinus luteus, Schulze (4) found asparagin, 

 phenyl-alanin, amido-valeric acid, arginin, cholin, and xanthin-like sub- 

 stances; in Cucurbita pepo, glutamin, asparagin, leucin, tyrosin, arginin, 

 cholin, vernin, and xanthin-like substances; in Vicia sativa, asparagin, 

 phenyl-alanin, leucin, amido-valeric acid, giianidin, cholin, and betain. Of 

 these, asparagin, glutamin, and arginin occur most widely and plentifully. 



The amount of amido-nitrogen present in rice, rice polish- 

 ings, beans, and mongos was found to bear a very close relation 

 to the amount of phosphorus pentoxide present in those sub- 

 stances, as may be seen from Table III. 



Table III. — Relationship between percentage of nitrogen and amido- 

 nitrogen in rice and beans. 



Article. 



Highly milled rice. No. 1 

 Highly milled rice. No. 2 

 Rice polishings. No. 1 . _ _ 

 Rice polishings. No. 2 ... 



Navy beans 



Mongos 



Total 

 nitrogen. 



Per cent. 

 1.43 

 1.22 

 5.40 

 5.10 

 4.13 

 3.71 



Amido- 

 nitrogen. 



Per cent. 

 0.03 

 0.00 

 0.% 

 0.504 

 1.23 

 0.616 



