82 ANDREWS. 



a slight drop occurred. They nursed for six weeks and died within twenty- 

 four hours of each other, September 22 and 23. 



Although the puppies gained in weight they never became fat and were 

 in fact rather lean-looking. Nothing of importance occurred until the 

 fourth week when it was noticed that they had some difficulty in walking 

 or standing. The ankles of the front feet turned under them; they swayed 

 from side to side, and apparently could not control the muscles so as to 

 go just where they desired. As time passed, all these phenomena were 

 augmented and other symptoms appeared. The hind legs became more 

 seriously affected than the forelegs. The puppies sat on their haunches 

 and moved their legs as little as possible. On getting up they fell to one 

 side or the other and stumbled on their noses; apparently they had lost 

 control of most of the muscles of locomotion. This condition continued to 

 grow worse until death. During the fifth week it was noticed that they 

 were becoming anasmic. . Also during the last two weeks the front feet 

 became oedematous. 



Necropsy was performed about ten hours after death. The bodies of 

 the puppies were emaciated and the subcutaneous tissues were anaemic and 

 oedematous throughout. The peritoneal cavity contained a slight increase 

 in fluid. The heart was neither hypertrophied nor dilated. All the inter- 

 nal organs were anjemic and oedematous. The intestines contained a large 

 number of ascaris and hookworms. The faeces were dark colored and in a 

 few places in the«mucosa of the intestines haemorrhages had taken place. 



EXPERIMENT NO. 2. (PLATE II.) 



Two puppies, 4 days old, were given to Case III to nurse on Septem- 

 ber 26. The 1-month-old child had died one week previously of typical 

 infantile beriberi. The woman showed marked symptoms of beriberi; 

 numbness, anaesthesia, and formication of legs; shortness of breath, distinct 

 heart murmur, and loss of knee jerks and other reflexes. 



For the first three days the puppies lost weight. They then continued 

 to gain until the end. One died October 19 after nursing twenty-three 

 days, and the other October 22 after nursing twenty-six days. 



Both these puppies became plumper and apparently fatter than the 

 first two. Nothing of importance was noticed in either of them until the 

 14th of October when both began to show symptoms of weakness in the 

 legs. This grew worse until it seemed that the hind legs were practically 

 paralyzed. The puppies would rise up on their front feet and then fall 

 over. Their feet and legs became oedematous. The first one, which died 

 on the 19th, developed no further symptoms; but the second, living three 

 days longer, developed marked dyspnoea, and the legs became greatly 

 oedematous. It made no attempt to move its hind legs but dragged them 

 along. Toward the last it could not raise itself on its front feet. 



Necropsy. Puppy which died October 19. — The body tissues are oedem- 

 atous, and the muscles are pale. The heart is apparently normal. The 

 lungs are congested and osdematous. The spleen and liver are dark colored, 

 firm, and congested. The kidneys are pale. The intestines contain a few 

 hookworms. All tissues are very moist. 



Necropsy. Puppy which died October 22. {Plate II.) — The subcuta- 

 neous tissues showed marked oedema. The muscles are pale. Increase of 

 fluid in the peritoneal and pericardial sacs. The right heart is dilated 



