146 STRONG AND TEAGUE. 



inoculated later into guinea pig No. 5461 by the cutaneous method. The 

 animal remained healthy. Culture 20 C 5 shows very heavy white colonies 

 of a coarse bacillus; evidently not plague. 



Case 21. — Advanced case with bloody sputum, containing enormous 

 numbers of plague bacilli. Temperature 40° C, pulse 130. Marked 

 dyspnoea, much vapor arising from the mouth. 



Plate A exposed for two minutes at a distance of 11 centimeters. No 

 coughing during exposure. Surface of plate wet by the vapor from the 

 breath. Result after forty-eight hours: Fifty-eight colonies. Most of 

 these do not resemble colonies of the plague bacillus. Nine of the colonies 

 that might possibly be plague were inoculated on agar slants and studied 

 further as follows: 21 A 1 and 2 = cultures of bacilli that do not decolorize 

 by Gram; morphology not right for plague. 2lA3 = a coccus or cocco- 

 bacillus which decolorizes by Gram; 0.5 cese of this culture was inoculated 

 subcutaneously into mouse No. 12 and 1 cese cutaneously into guinea pig 

 No. 5482; both of these animals remained healthy. Culture 21 A 4 = a 

 bacillus which does not resemble the plague bacillus morphologically and 

 which only partially decolorizes by Gram's stain; 1 oese of this culture was 

 inoculated by the cutaneous method into guinea pig No. 5483; the animal 

 remained healthy. Cultures 21 A 5 and 6= large bacilli. Gram-positive. 

 21 A 7 = the same culture as 21 A 4. 21A8=the same bacillus as 21 A 3; 

 inoculated into guinea pig No. 5322 by the cutaneous method; this animal 

 remained healthy. 21 A9 = a bacillus whose morphology does not resemble 

 the plague bacillus; only partially decolorizes by Gram's stain; 1 cese 

 of this culture was inoculated by the cutaneous method into guinea pig 

 No. 5485; this animal remained healthy. 



Plate B exposed for two minutes at a distance of 11 centimeters. No 

 coughing during exposure. Result after forty-eight hours : A large surface 

 growth covers two-thirds of the plate, in which are situated 3 rather large 

 white colonies which do not resemble plague. Three small isolated colonies, 

 situated near the edge of the plate, might possibly be plague. Microscop- 

 ically, these colonies are made up of a small bacillus which only partially 

 decolorizes by Gram's stain. One of these colonies was transplanted to 

 an agar slant and later inoculated into guinea pig No. 5479 by the cutaneous 

 method; this animal remained healthy. 



SERIES VII. 



Case 25. — Advanced case with much bloody sputum, containing enormous 

 numbers of plague bacilli. Patient died two hours after exposure was 

 made. Plates exposed for two minutes at a distance of 15 centimeters 

 from the mouth. 



Plate A. Patient coughed four times during exposure. Result after 

 twenty-four hours: Twelve colonies. After forty-eight hours, 33 colonies. 

 All of the colonies, which looked at all suspicious of plague, planted on 

 agar slants Nos. I to IX. A suspension was then made with 0.5 centimeter 

 of peptone solution of all the colonies on the plate and 4 cesen of this 

 suspension rubbed over the scarified abdomen of guinea pigs Nos. 8 and 

 15 respectively. Guinea pig No. 8 was found dead less than twenty-four 

 hours after inoculation. There were no evidences of plague infection. 

 Guinea pig No. 15 was found dead four days after inoculation. At the 

 necropsy there were inguinal hsemorrhagic buboes and a typical plague 

 spleen. Innumerable plague bacilli were present in the buboes and spleen. 



