148 STRONG AND TEAGUE. 



agar-slant-cultures. One-fourth of an (sse made up of several of the 

 small colonies on the plate was inoculated into mouse No. 16 and 0.25 oese 

 of several of the other colonies into mouse No. 17, both subcutaneously; 

 both of these animals died after forty-eight hours with marked swelling 

 of the inguinal glands which contained innumerable plague bacilli; in 

 each the spleen was swollen and contained innumerable plague bacilli; 

 in each, cultures from the heart showed Bacillus pestis. Results of 

 colonies transplanted previously from the plate to agar slants are as 

 follows: Culture 28 C 1, a very short bacillus which resembles the pest 

 bacillus morphologically and takes a bipolar stain; does not decolorize by 

 Gram; 0.5 oese inoculated into mouse No. 21; the animal did not develop 

 plague infection. Culture 28 C 2, short bacillus which does not decolorize 

 by Gram's stain. 28 C 3, evidently the same organism as 28 CI. 28 C 4, 

 a coccus or very short bacillus which does not decolorize by Gram; 

 evidently the same as 28 CI. 28 C 5, colonies suggest those of Bacillus 

 pestis; morphologically, a short bipolar staining organism; later in- 

 oculated into guinea pig No. 5457 by the cutaneous method; this animal 

 died of typical plague infection six days after inoculation. 28 C 6, a coarse 

 bacillus, evidently not plague. 28 C 7, evidently the same organism as 

 28 C 1, not plague. 28 C 8, 9, and 10, a bipolar organism which decolor- 

 izes by Gram's stain; 0.5 oese of 28 C 10 was later rubbed over the shaved 

 abdomen of guinea pig No. 5458, which died of typical pest infection 

 five days after inoculation. 



Plate D. Patient coughed several times during exposure. Plate ex- 

 posed at a distance of about 70 centimeters and only during the time of 

 coughing. Result after forty-eight hours: A large surface growth covers 

 about three-fourths of the plate. In this are situated about 50 colonies 

 which might be plague colonies. Outside of this growth are situated 

 3 colonies which were . planted on agar slants. Examined microscopically, 

 the organism from these cultures is a very large bacillus which does 

 not decolorize by Gram, but the culture does not look pure and in it 

 there appear to be a few smaller bacilli which decolorize. For this 

 reason, 1 oese of 28 D 1 and 1 oese of 28 D 2 were inoculated into guinea 

 pigs Nos. 5454 and 5323 by the cutaneous method; both of these animals 

 remained healthy; evidently the culture did not contain the plague bacillus. 



Plate E exposed for one-half a minute at a distance of about 70 

 centimeters from the mouth of the patient. Patient coughed several 

 times during exposure. Result after forty-eight hours: About 100 colonies 

 are scattered through a large surface growth which covers the entire 

 plate. Since it is impossible to isolate the colonies, the whole, plate was 

 suspended in a few drops of saline solution and several oesen rubbed over 

 the scarified abdomen of guinea pig No. 9; this animal was found dead 

 four days later. There were marked inguinal buboes on both sides and 

 the spleen showed miliary abscesses; innumerable plague bacilli were 

 present in smears from the spleen and buboes, and Bacillus pestis was 

 isolated from the heart. 



Plate F exposed for two minutes at a distance of about 5 centimeters. 

 No coughing during exposure. Result after forty-eight hours: Eight col- 

 onies. Only 5 of these could possibly be plague. These were planted on 

 agar slants. Only 3 of them looked at all like plague colonies. 28 F 1, 

 2, and 4 might be plague colonies; however, a microscopical examination 



