150 STRONG AND TEAGUE. 



SERIES X. 



Case 32. — ^Advanced case, temperature 39°. 2 C, pulse 110. Pulmonary 

 oedema of both lungs; much bloody sputum, containing innumerable plague 

 bacilli. 



Plate A exposed for two minutes at a distance of 11 centimeters. No 

 coughing during exposure. Talked slightly. Result after forty-eight 

 hours: A surface growth covers the entire plate. It was, therefore, im- 

 possible to study any isolated colonies beneath this. The whole plate was, 

 therefore, suspended in a few drops of peptone solution and mouse No. 23 

 inoculated with several oesen subcutaneously. This animal remained 

 healthy. 



Plate B exposed for two minutes at a distance of 11 centimeters. No 

 coughing during time of exposure. Patient breathing quickly. Result 

 after twenty-four hours: Plate sterile. After forty-eight hours, a heavy 

 growth covers most of the plate, around the edge of which are a few 

 delicate colonies measuring from 1 to 2 millimeters in diameter. Micro- 

 scopical examination of these colonies shows a short bacillus which only 

 partially decolorizes by Gram's stain; 2 (Bsen of a suspension of these 

 colonies were inoculated subcutaneously into mouse No. 24, which did not 

 develop plague infection. 



Plate C. Patient coughed slightly about ten times during exposure. 

 Result after forty-eight hours: Surface growth over four-fifths of the 

 plate. A few small colonies in this growth. Four or 5 pin-point colonies 

 outside the growth. Twenty-four hours later the whole plate covered by 

 growth. Plate discarded. 



Plate D. Exposed at a distance of about 70 centimeters. Patient gave 

 one good cough during time of exposure which was for about one-fourth 

 of a minute. Result after forty-eight hours: Surface growth over four- 

 fifths of the plate. Three colonies outside of this which might be plague. 

 Transplanted to agar slants. Results as follows: 32 D l = a bacillus which 

 decolorizes by Gram; morphologically, it looks rather thick for the plague 

 bacillus, though from the colony, the organism might be the plague bacillus ; 

 inoculated later into guinea pig No. 5318 by the cutaneous method; the 

 animal died seven days after inoculation with typical plague infection. 

 32 D 2 = a bacillus which does not decolorize for the most part; however, 

 there are a few organisms which are smaller and decolorize by Gram's 

 stain; culture does not appear to be pure; 1 cese of this impure culture was 

 later inoculated into guinea pig No. 5446 by the cutaneous method; the 

 animal died of typical plague infection four days after inoculation; there 

 were typical inguinal buboes and beginning miliary abscesses of the spleen. 



Plate E exposed at a distance of about 30 centimeters for three-quarters 

 of a minute. Patient gave one good cough during exposure. Result after 

 forty-eight hours: Two irregular-shaped colonies that could not be plague; 

 otherwise plate sterile. After seventy-two hours about 32 colonies, pin- 

 point and pin-head in size scattered over the plate. Six of these colonies 

 were planted on agar slants; microscopical examination of all revealed a 

 bacillus which took a bipolar stain and decolorized completely by Gram's 

 stain. Two of these agar-cultures were inoculated later by the cutaneous 

 method into guinea pigs Nos. 5311 and 5447; the former died nine days 

 after inoculation and the latter ten days after inoculation, both of typical 

 plague infection with miliary abscesses in the spleen. 



Plate F exposed for two minutes at a distance of about 70 centimeters, 



