264 



BARBER AND TEAGUE. 



gauze were boiled and while still warm were squeezed out and applied 

 loosely over the lower portion of the face from the eyes to below the chin. 

 The gauze was not in layers but was placed irregularly as in surgical dress- 

 ings which are intended to absorb pus. A many-tailed bandage with holes 

 for the eyes, such as is used with the Mukden mask, pressed the moist 

 gauze firmly against the face and held it snugly in place. This mask was 

 about five or six centimeters thick over the mouth and became thinner 

 toward the edges. Goggles were worn by this boy also and the top of his 

 head was covered with a cloth reaching down to the mask. 



Boy No. 1. Mukden mask. 

 Saliva taken before exposure 



[Plate No. 1 : Prodigiosus absent. 

 I Plate No. 2: Prodigiosus absent. 



Cotton from nostrils before exposure Prodigiosus absent. 



Cotton within mask after exposure 



Cotton before mouth after exposure 



Cotton from nostrils after exposure 



Plate held by boy during exposure 

 Boy No. 2. 



Saliva taken before exposure 



[Plate No. 1: Prodigiosus present. 



[Plate No. 2: Prodigiosus present. 



[Plate No. 1 : Prodigiosus present. 



[Plate No. 2: Prodigiosus present. 



[Plate No. 1 : Prodigiosus present. 



[Plate No. 2: Prodigiosus present. 



4,420 prodigiosus colonies. 



Broquet mask. 



[Plate No. 1 : Prodigiosus absent. 

 [Plate No. 2: Prodigiosus absent. 



Saliva taken before exposure 



Cotton from nostrils before exposure Prodigiosus absent. 



_, ^^ , , ^1. ^j. [Plate No. 1 : Prodigiosus absent. 



Cotton before mouth after exposure im ^ -kt « -r. j- • ^ 



[Plate No. 2: Prodigiosus absent. 



„ ^^ . ^ ., .. [Plate No. 1: Prodigiosus absent. 



Cotton from nostrils after exposure i™ ^ xt r, t^ ■,■ ■ ^ 



[Plate No. 2 : Prodigiosus absent. 



Plate held by boy during exposure 4,000 prodigiosus colonies. 



Boy No. 3. Mask of wet gauze. 



[Plate No. 1 : Prodigiosus absent. 

 jPlate No. 2: Prodigiosus absent. 



Cotton from nostrils before exposure Prodigiosus absent. 



^ ,^ , „ ^-L j,j. (Plate No. 1: Prodigiosus present. 



Cotton before mouth after exposure -^t^i , ^t r, r, j- ■ j. 



[Plate No. 2 : Prodigiosus present. 



„ ^^ „ J. -1 ^x [Plate No. 1 : Prodigiosus present. 



Cotton from nostrils after exposure -It^i^-kt r, t^ ^^ ■ ±. 



I Plate No. 2: Prodigiosus present. 



Plate held by boy during exposure 4,485 prodigiosus colonies. 



DISCUSSION OF PROTOCOL NO. 4. 



In spite of the long interval (two hours) which elapsed be- 

 tween the spraying and the exposure of the subjects, this test 

 must be regarded as a very severe one, for the plates show that 

 numerous living prodigiosus bacilli still remained suspended in 



