50 MIDLAND NATURALIST. 
Mycological Notes. * 
Guy WEsT WILSON. 
I. ZYGOSPORES OF MUCOR STOLONIFER. 
In the course of a series of cultures of various species of Mucor 
there was obtained a number of zygospores of 77. stolonifer Ehrenb. 
( Rhizopus nigricans Ehrenb.) These appeared the latter part of 
April, in a stender dish culture on a decoction of rye bread. Except 
for size they were typical of the species; but measured only 80-90, 
or about one-half the size usually recorded. Innoculations from 
this culture to bread were made with the results that a good growth 
of mycelium was formed and numerous gametes produced. From 
some cause, the nature of which was not determined, very few 
zygospores were produced, and these were none of them strictly 
normal. While no cytological study was made, a careful gross 
examination of the protoplasmic contents of both cultures was 
made. In the first culture the zygospores were completely opaque, 
black in appearance, and upon rupture of the epispore a very dis- 
tinct oil drop was visible. In the second culture, even the 
apparently matured zygospores in addition to being smaller in size 
* The observations upon which these notes are based were made w 
student in the laboratory of Dr. J. C. Arthur at Purdue in 
La Fayette, Ind. 
