AGRICULTURAL EXPERIMENT STATION. 207 



weather shows that oviposition continues through the season. This 

 is confirmed by an examination of females taken about the middle 

 of September. In them were found plenty of mature eggs and 

 others in varying stages of development. 



Eggs and Reproductive System. 

 Discovery of the eggs in the apple, and dlso in the ovary of the 

 female, completes our knowledge of the life changes of Trypeta. 

 The researches of 1888 made us familiar with the entrance punctures. 

 These were again found in the first lot of apples examined early in 

 July, 1889. The first puncture examined contained one egg inserted 

 vertically beneath the skin, and entirely concealed in yellowish, 

 withered tissue. It was described and figured. The second apple 

 revealed another egg, which was photographed. A third egg was 

 transferred to a live box in apple juice and watched. In about fif- 

 teen minutes a motion was observed in the end opposite the pedicil. 

 Soon the shell burst irregularly, the head of the larva protruded, and 

 in less than a minute it had crawled out. A careful examination 

 under high power proved it to be the larva of Trypeta. We went 

 to Cumberland Centre the next day to study the insect at home. 

 Caught several pairs copulating, and made a careful microscopical 

 examination of the reproductive system. Found eggs in great 

 numbers, which agreed exactly with those found in the apples 

 During the season hundreds of eggs were taken from the females 

 and many temporary and a few permanent slides prepared and 

 additional photographs made. We believe no one will dispute our 

 claim to discovery of the eggs, as nothing has heretofore been 

 written about them. 



Nature of the Reproductive System. 

 We find no record of a histological examination of this part of 

 the insect. The oviduct leading into the ovipositor is short and 

 soon divides into two quite long convoluted tubes, oae on each 

 side, leading to the ovate ovaries, which nearly fill the abdominal 

 cavity. Each half ovary contains about twenty-four chains of 

 eggs, each chain having at least seven eggs attached together, in 

 different stages of development, which would make the number of 

 eggs at least 336. As many as eleven stages in the development 

 of the eggs were observed. As many as seven stages were fre- 

 quently seen attached and we are not prepared to say but what the 

 loose forms seen in the reproductive passages were also detached 



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