AN ENDOMYCES FROM APPLE. 49 



The characters of the growth upon a number of culture media 

 will be given the temperature being about 20° C. in each case. 

 The notes are based on observations of 2 or more tubes in every 

 case, and with the agars on plate cultures as well. 



Prime agar. When conidia are sown in plates of prune agar 

 they germinate readily and by the end of 48 hours a much 

 branched mycelium is formed with numerous conidiophores 

 which bear large numbers of conidia. For good growth the 

 medium must be acid. Prune agar was made alkaline to — 2.5 

 Fuller's scale with NaOH, neutral, and acid to +2.5, +10, and 

 +20 with HCl. There was no growth in the alkaline medium 

 and very little in the neutral while the growth in the acid me- 

 dium was good, that in +10 and +20 being equally good. The 

 relation of the growth of the fungus to acids will be discussed 

 later in this paper. 



Prune decoction + 12. The growth in a decoction made by 

 cooking 6 good sized prunes in 1000 c. c. of distilled water is 

 very good. The mycelium develops rapidly in the bottom of 

 the tube and after about 48 hours a film has developed over the 

 surface of the liquid. The spore sacs or asci develop well in this 

 medium and many are found which have formed spores at the 

 end of 10 days. There is a good development of the mycelium 

 and there is no tendency to break down to form oidia in the 

 liquid medium as is the case with Endomyccs Magnusii. 



Potato agar + 6. At 42 hours conidia had been produced in 

 large numbers. After 5 days a few developing spore sacs were 

 observed and when the cultures were 9 days old large numbers 

 of spore sacs were seen, in some of which the spores had de- 

 veloped. 



Potato brotli. In neutral or alkaline potato broth there was 

 no growth but when a drop of lactic acid was added to each 

 tube before inoculation the fungus grew readily and at about 

 the same rate as in prune decoction. Large numbers of asci 

 were found 9 days after the tubes were inoculated. 



Bean agar. The growth here is practically the same as on 

 potato agar. 



Bean f^ods. Tn bean pod tubes the growth is mostly confined 

 to the liquid and to that part of the pod just above the liquid. 



J'egetable plugs. On sterilized jiotato, beet, turnip, and car- 



