50 MAINE AGRICULTURAL EXPERIMENT STATION. I9IO. 



rot cylinders in tubes the growth is good. In 40 hours the en- 

 tire slant of the plug is covered bv the fungus and conidia are 

 being formed. The conidia are formed in such abundance as 

 to become piled up in considerable masses giving a wet slimy 

 appearance. After conidia are formed, they germinate in some 

 cases, producing short germ tubes on which conidia are formed 

 in the irregular manner described as occurring in hanging drops. 

 The vegetable plugs seem to be much more favorable to the 

 development of conidia than asci, as the asci are not found in 

 very large numbers. 



Apple zvood. On sterilized apple twigs in tubes the growth 

 is good. There is considerable mycelium in the liquid and a 

 thick slimy covering on the upper ends of the pieces of wood at 

 the end of one week. Both conidia and asci are produced in 

 large numbers. 



Beef extract agar. It has been noted above that the spores 

 did not germinate in hanging drops of neutral beef extract 

 broth. On neutral beef extract agar no growth took place but 

 wdien the agar was made acid to +20 with hydrochloric, lactic, 

 or acetic acid the fungus grew to a slight extent. 



Beef extract gelatin. On this gelatin, when made acid with 

 lactic acid, the growth is about the same as on beef extract agar. 

 No liquefaction of gelatin but there was very little growth. 



Beef extract gelatin + dextrose. The fungus grows very 

 well on this medium and causes some liquefaction of the gelatin 

 at the end of one week. After one month the gelatin in the 

 tubes was hquefied to a depth of 1.5 cm. 



Sugar agars. Agars made by adding i per cent of dextrose, 

 saccharose, lactose or mannite to beef extract agar. When these 

 sugar agars were neutral, no growth took place, but when they 

 were made acid the fungus grew on all of them. The growth on 

 lactose and mannite is poor but on dextrose and saccharose the 

 growth is good. The fungus produces a rather thick, whitish 

 mass composed largely of conidia but does not form many asci. 



Sugar broths. The same 4 sugars which were used in agars 

 were also added to beef extract broth. One, 2, 5 and 10 per 

 cent of dextrose were tried and gave about equally good growth. 

 With the other sugars 2 per cent was added to the broth in 

 each case. These broths were made acid to +15 with hydro- 



