AN ENDOMYCES FROAI APPLE. 5 1 



chloric acid. The relative growth with the sugar broths is the 

 Faiiic as with the sugar agars. Dextrose and saccharose give 

 much better growth than lactose and mannite. Few asci de- 

 velop in these broths in comparison with the number that de- 

 velop on some other media. 



Diinhani's solution. Small amount of growth in this medium 

 and in the same medium plus lactic acid. 



Dunham's solution + sugars. When the 4 sugars used with 

 agar and beef broth were added to Dunham's solution which 

 had been made acid with lactic acid they gave about the same 

 relative growth as*with the agars and beef broth. 



Glycerine agar. Five per cent glycerine added to beef ex- 

 tract agar and made acid with lactic acid. The growth is thick 

 and somewhat wrinkled when the cultures are 5 days old. When 

 a small piece was removed with a needle, it was found to be 

 rather gelatinous and hard to tease apart. When examined 

 with the microscope this material showed a very great number 

 of asci. This medium seemed to be more favorable to develop- 

 ment of asci than any of the other media which were used. 



Rice. The fungus does not grow very well on rice sterilized 

 in distilled water but it forms a considerable number of asci. 



Corn. Enough crushed yellow dent corn was placed in each 

 tube so that when distilled water was added to soak it up it 

 made about 10 c. c. The fungus grew very well on this medium 

 and produced both conidia and asci. 



Milk. Fresh milk from which the cream had been separated 

 was sterilized in the Arnold sterilizer. The fungus did not 

 grow well in this medium but when a drop of lactic acid was 

 added to each tube before inoculation, good growth took jilace. 



Ref^ation of Growti-i to Acids. 



It has alreadv been noted in several places in connection with 

 tlie growth on various media that this fungus reqm'rcs an acid 

 medium for its growth. A considerable amount of work has 

 hccn (lone and data obtained on this subject. The fungus has 

 l)ceu grown in liquid media to which definite amounts of di<¥cr- 

 cnt acids have been added. For the most part. 2 per cent, dex- 

 trose broth has been used because it was found that this made 

 a favorable medium. An attempt has been made to determine 



