20. 1909 Apr 



il 12, 



1909 



+27.. 5 







26, 1909 



13, 



1909 



+39 



April 2?. 1909 



+40 



26. 1909 



13, 



1909 



+51 



" 27. 1909 



+48 



26, 1909 



13, 



1909 



+50 



" 27. 1909 



+55 



26, 1909 









•' 27. 1909 



+41 



26, 1909 



12, 



1909 



+49 



•' 27. 1909 





26, 1909 









■' 27, 1909 



+58 



26, 1909 



12, 



1909 



+78 



'• 27. 1909 



+77 



26, 1909 



12 



1909 



+95 



" 27. 1909 



+95 



26, 1909 



26, 



1909 



+100 



■' 27. 1909 



+100 



26, 1909 



26, 



1909 



+112 



" 27. 1909 



+110 



29, 1909 









'• 27. 1909 



+145 



29, 1909 









" 27. 1909 



+180 



29. 1909 









" 27. 1909 



+245 



29, 1909 









•' 27, 1909 



+311 



AN ENDOMYCES FROM APPLE. 53 



Acid used. Acidity. Inoculated. Titrated. Acidity. Titrated. Acidity 



Hydrochloric +10 Marcli 20. 1909 

 +20 

 +30 



-HO 



Lactic +20 



+30 



-HO 



+60 



+80 



+90 

 +100 

 +120 

 +150 

 -1-220 

 +280 



There is considerable difference in the amount of dift'erent 

 acids which can be added to 2 per cent dextrose broth before 

 the growth of the fungus is checked or entirely prevented. With 

 hydrochloric, sulphuric and nitric acids there is good growth up 

 to and including +60 but no growth at +80. Of the organic 

 acids used, it requires a smaller amount of formic acid than of 

 any of the others to prevent growth. There was fairly good 

 growth at +20 and at +30, very little at +40 and none at 

 +50. Malic, lactic, citric, and tartaric acids give good growth 

 to high acidities. Very little difference can be noted with these 

 4 acids up to and including +300. Malic acid was only tested 

 to +350 but gave good growth at that acidity. Lactic acid gives 

 a little poorer growth at +350 than at +300, at +400 there is 

 very little growth and at +450 it can scarcely be detected. Cit- 

 ric acid gave good growth up to +500 and was not tested fur- 

 ther. Tartaric acid gives good growth up to +200. above +200 

 growth takes place slowly but there is a small amount of growth 

 up to and including 4-6oo. With oxalic and acetic acids the 

 growth increases slowly in comparison with that in citric acid 

 for example. The limits of growth with oxalic acid is between 

 + 100 and +T50. With acetic acid there is very little growth 

 at +200 and none at +250. 



