AN ENDOMYCES FROM APPLE. 55 



time to time from prune agar and prune decoction cultures in 

 both of which the fungus produces both conidia and ascospores. 

 These cuhures were kept at the temperature of the laboratory. 

 It was found that growth took place readily when transfers were 

 made from prune agar cultures up to 5 months old provided the 

 agar had not become dried out. In no case was growth obtained 

 from agar tubes in which the agar was dry. Growth took place 

 when transfers were made from prune decoction cultures 6 

 months old. At that time, the liquid was almost all evaporated 

 and the tests were not carried further. 



Endoniyces Magnusii. 



A culture of this fungus was secured from the Association 

 Internationale des Botanistes and has been grown upon a number 

 of the same culture media under the same conditions for com- 

 parison. This species has been studied by Ludwig (10). Bre- 

 feld { i), and Hansen (6), all of whom grew the fungus in cul- 

 ture on a number of media. Their accounts agree for the most 

 part. A branched mycelium is produced, the cells of wliich 

 separate very readily to form oidia. Brefeld found that when 

 the mycelium was grown in such a way that it was buried by the 

 culture medium it did not break down so readily to form oidia 

 and asci were formed. Hansen did not find the asci in any of 

 his cultures, but his description of the mycelium and oidia agrees 

 with tliose of Brefeld and Ludwig. 



In this study, Endouiyces Magnusii has been grown on the 

 following culture media : Prune agar, potato agar, bean agar, 

 prune decoction, bean pods, potato, turnip, carrot and beet 

 cylinders, sterilized oak wood, i per cent dextrose, saccharose, 

 lactose, and mannite agars, 2 per cent broths of the same 4 

 sugars, 2 per cent dextrose gelatine, 5 per cent glycerine broth, 

 5 per cent glycerine agar, and in both alkaline and acid inir.;. 

 Tlu' fungus grew well in all of these media except in man- 

 nite, lactose, and glycerine agars and broths and alkalin: milk. 

 When tlu' milk was made acid, the growth was good. In 

 all (if the media wliicli were used, the fungus goes through 

 the same stages, l-'irst, there is a development of mycelium 

 which \Qvy soon breaks down to form oidia. After a little 

 time, the oidia become (|uite thick walled and in some cases 

 almost spherical. In none of these cultures have asci been 

 found althi:ugh thev have been searcheil for repeatedly. 



