AN ENDOAIYCES FROM APPLE. 6l 



"with iodine in potassium iodide, the mycelium, conidia and the 

 very young asci are not stained, but asci which have begun to 

 swell out into the spherical form and those which are older are 

 stained brown. The ascospores are also stained brown by the 

 iodine. Schioning has observed the same in the case of 

 Saccharoniycopsis capsniaris Schioning. 



Systematic. 



As was stated in an earlier part of this paper, if we classify 

 this fungus according to the classification given by Schroter 

 (12) it would be placed in the genus Endomyces, and it has also 

 been shown that this species differs from any of the described 

 species of that genus. There would be another possibility in its 

 classification and that is that it might belong among the fila- 

 mentous forms of the Saccharomycetaceae. The classification 

 of the Saccharomycetaceae has been discussed by Hansen (7) 

 and by Guillermond (4). Miss Stoppel (15) also gives briefly 

 the characters of the genera and also the characters of En- 

 domyces in her paper on Eremascus fertilis. 



The relationship between the Saccharomycetaceae and the 

 Endomycetaceae seems very close. Such a form as Saccharoniy- 

 copsis capsularis, for example, which has a well developed 

 mycelium, and in which cells of the mycelium develop into asci 

 bears a very close relation to an Endomyces in which the asci are 

 formed on short liranches from cells of the mycelium. 

 Saccharomycopsis however, produces yeast like cells which mul- 

 tiply by budding, Schioning (13) in his study found that it was 

 possible to cause this fungus to develop in that way with almost 

 no production of mycelium in certain culture media and that, 

 on the other hand, in certain other culture media there was a 

 good development of mycelium and not much of the yeast form. 

 None of the species of Endomyces develop typical yeast like 

 forms in culture. In Endomyces deeipiens and Endomyces 

 Magnnsii the cells of the mycelium separate as oidia but there 

 is no yeast like budding. In the fungus from apple described 

 in this paper, conidia are produced on short conidiophores. and 

 in some cases in liquid media, the conidia develop from short 

 germ tubes as shown in Fig. 67. There is, however, no typical 

 yeast like ])U(.lding as the writer iniderstands that term. 



The Endomycetaceae cannot be separated from the Sacchar- 

 omycetaceae on the basis of fermentation because in both fam- 



