62 MAINE AGRICULTURAL EXPERIMENT STATION. I9IO. 



ilies there are species which cause fermentation of certain sug^ars 

 and other species which do not cause fermentation. Endoniyces 

 jiagiiusii and Endoniyces fibuliger Lindner (9) cause fermen- 

 tation. 



On the basis of a sexual fusion of cells or of nuclei before the 

 formation of endospores there is no reason for placing the 

 Saccharomycetaceae in the Hemiascomycetes and the Endomy- 

 cetaceae in the Euascomycetes because in both families there are 

 species in which the ascus develops from such a fusion and 

 other species in which the ascus develops without such fusion. 

 Guillermond (5) gives a good discussion of this point with re- 

 gard to the Saccharomycetaceae and shows that in some of them 

 two vegetative cells fuse before the ascus is formed, in others 

 there is no such fusion but the ascospores fuse in pairs upon 

 germination ,and in some others as Saccharomycopsis, there is 

 neither a fusion of cells before the formation of the ascus nor 

 a fusion of the spores upon germination. In the Endomy- 

 cetaceae, according to Schroter, there is included one genus, 

 Ereinascns, in which there is a fusion of cells before the ascus 

 develops. In Endoniyces Magniisii, there are some cases in 

 which there is a fusion of cells before the ascus develops 

 and other cases in which the ascus develops from a sin- 

 gle branch from a cell of the mycelium. Whether there is a 

 fusion of nuclei in this species has not been determined. In 

 the other species of Endoniyces the ascus develops from a sin- 

 gle branch but it has not been determined wdiether there is a 

 fusion of nuclei before the ascospores develop. 



In the fungus which has been described in this paper, the 

 ascus develops from a single branch from a cell of the mycelium 

 and a single nucleus passes into the young ascus. There is no 

 fusion of germ tubes at the time of germination. We have here 

 then a fungus wdiich corresponds with those yeasts in which 

 there is no sexual fusion of cells either before or after forma- 

 tion of ascospores. So far as the development of mycelium or 

 the formation of ascospores is concerned there would seem to 

 be little basis for placing a fungus like Saccharomycopsis cap- 

 siiJaris in the Hemiascomycetes and the fungus described in this 

 paper in the Euascomycetes. However, the fact that this fungus 

 does not reproduce by typical yeast like budding would prevent 

 its being classified in the Saccharomycetaceae. It has seemed 

 best to the writer, therefore, to classify it as a new species of 

 the genus Endoniyces. 



