AN ENDOMYCES FROM APPLE. 63 



Whether the spore sacs of the yeasts or of Endouiyces should 

 be regarded as true asci has been questioned. Brefeld regards 

 the spore sacs in yeasts as sporangia and he considers such 

 forms as Endomyces to be more highly developed than the 

 yeasts because the number of spores in the spore sac has become 

 more definitely fixed than in the yeasts. There are, however, 

 certain species among the yeasts in which the number of spores 

 seems to be as definite as in Endomyces. 



Harper (8) has shown that the spore formation in asci dif- 

 fers from that in sporangia and that the young ascospores are 

 cut out of the cytoplasm in such a way that they are surrounded 

 by epiplasm while this is not the case in sporangia. Guiller- 

 mond (5) has shown that the young ascospores in certain yeasts 

 are surrounded by epiplasm, and Miss Stoppel (15) has shown 

 that the same is true in Bremascus fertilis. In the Bndomyccs 

 described below the writer has found the same condition. It is 

 desirable that further cytological study should be made upon 

 species of the Hemiascomycetes and upon the lower forms of 

 the Euascomycetes as it seems probable that such study will lead 

 to a clearer understanding of the relationship of these forms to 

 each other and to the higher Ascomycetes. 



Description of Species. 



Endomyces mail n. sp. Branched mycelium with cross walls 

 develops in a large number of culture media, conidia averaging 

 3x8 microns formed on short conidiophores or on the ends of 

 short germ tubes, no typical yeast like budding, asci 11-14 mic- 

 rons in diameter, usually formed singly on short side branches 

 of the mycelium without fusion of cells or nuclei, ascospores 

 almost spherical but slightly elongated 4.5 x 5.5 microns, 

 thickened places on walls, brown when mature. Fungus grows 

 well in large number of culture media, in liquid media, as prune 

 decoction, a pellicle is formed in one to 2 days composed of 

 mycelium and conidia, mycelium in liquid as well as at surface, 

 asci produced in 5-8 days both at surface and in liquid. Cultures 

 take on a Ijrownish color after ascospores are formed. No fer- 

 mentation with formation of CO., in dextrose, saccharose, lac- 

 tose, mannite or glycerine broth. Requires acid culture media 

 for growth. 



Found in decaying fruit of apple, Orono, Alaine, causes small 

 amount of decay of ripe apples. 



