212 MAINE AGRICULTURAL EXPERIMENT STATION. I9IO. 



At the time of preparation of this paper I have six slides of 

 this species lent me by Mr. Sirrine. One slide is labeled "Tetra- 

 neiira iilmi, winged adult, and pupa and stem mother. From 

 cocks comb gall on Uhnus americana. I. A. C. 6-27-'93." The 

 specimens accord with Maine specimens of Tetraneura colo- 

 phoidea, and the winged forms accord with a slide of T. gra- 

 niinis lent me by Mr. Monell. The Sirrine material also com- 

 prises Tetraneura graminis, larvae, pupse, and winged adult 

 collected from bladtes of grass, Leersia virginica and larvae from 

 roots of same. Also migrants which had alighted on the bark 

 of Uhnus americana and their progeny, true males and females 

 (see figures) and an egg. The collections from grass were 

 labeled "I. A. C. g--^o-g2' and "I. A. C. 9-i3-'93." The speci- 

 mens collected from elm trunk are dated "I. A. C. 10-4-^2." 



A careful consideration of the evidence at hand and the com- 

 parison of the identical material recorded by Osborn-Sirrine 

 with Maine material of colophoidea and the comparison of it 

 all with the Monell slide of Tetraneura graminis convince me 

 that these are all the same species. The evidence of the return 

 migration and the structural evidence certainly seems sufficient 

 to establish their identit}^ Moreover the experimental evidence 

 in the following letter gives interesting testimony. 



RiVERHEAD, N. Y., March 14, loio. 

 My Dear Miss Patch: — 



Replying to your letter regarding the colonization of Tetraneura and 

 Colopha; I succeeded in colonizing Tetraneura on Leersia and Colophtt 

 on Eragrosfis. The transfers were made by transplanting the grasses 

 to pots and growing same under lantern-globes in forcing house. As 

 soon as the migrants in the galls showed winged specimens, the galls 

 were placed on the pots under the jars allowing the migrants to find the 

 host plants themselves. Galls containing Tetraneura were placed under 

 jars containing Leersia, also under jars containing Bragrostis. Galls 

 containing ColopJia were treated in same way. 



Unfortunately the larvae of the migrants were not carried to maturity 

 in confinement, nor the number of summer broods on annuals deter- 

 mined. At the time I had class work and other field work to look r.fter. 

 Those who had charge of the forcing house neglected to ventilate one 

 hot day and I found things cooked. 



The two species were found in field at same time and frequently dur- 

 ing the summer; but if you can carry them through the summer in con- 

 finement and determine number of broods, do so. 



Sincerely yours, 



F. A. SIRRINE. 



