— 115 — 



dung must be slightly moistened with water, but excess of moisture 

 greatly hinders the development of the fungi. 



The dimensions of the vessel, in which the culture was produ- 

 ced, had a distinct influence on the time of the appearance of 

 the fruit bodies. Generally Koch's cups were used (3 c/m. high 

 and 4 c/m, in diameter). The perithecia developed always first 

 on the walls of the vessel and then on the nourishing medium. 

 When, instead of the said cups, were used Petri cups (9 c/m. in 

 diameter) the development of perithecia was delayed to about 8 to 

 10 days (same notes Ternetz 1900, who cultured Ascophanus). 



The presence of a small number of bacteria, not only did not 

 hinder, but contributed to the development of the fruit bodies. If 

 for the better clearing from the bacteria one regrafted the myce- 

 lium in a larger quantity than indicated on page 113, the cultures in 

 the end did not produce asci fruitfulness, and generally too great 

 a cleaning of this culture results in a considerable diminuation of 

 the number of developing perithecia. And so one must notices a 

 somewhat favorable influence of the bacteria on the development 

 of these fungi. Cultures, with a small admixture of bacteria, de- 

 velop very Avell, not worse, but even better, than in ordinary, na- 

 tural conditions. A perfectly pure culture in our case was obtained 

 only once. The ' experiment was made on P. fimiseda. By means 

 of a great number of regraftings the mycelium was quite cleared 

 of bacteria. In the result (which are the same as described by 

 Moiliard for the Ascobobus 1903), not only the perithecia, but 

 even the ascogonia did not develop at all. As to the hyphae, one 

 noticed quite a wild growth. Part of it, rising from the surface of 

 the substratum into the air, developed into a delicate downy, sil- 

 very veil, and another part formed an especially very dense tissue. 

 It spread over the substratum, rose along the walls of the 

 glass vessel and even crawled from under the cover of the cup 

 into the open ^). 



It is interesting to note the influence of the temperature on the 

 fruiting of these fungi. Two types were here distinguishable. For 

 some species high temperature detained the formation of the pe- 



1) We observed the same results, лгЬеп the pure culture of Ascobolus immer- 

 3US was obtained. 



