— ЦП — 



rithecia, for others, on the contrary, it hastened it. To the first 

 type belong, P. fimiseda, P. coprophila. P. setosa and Sporormia. 

 Their cultures, kept two weeks in a cupboard under a constant 

 temperature of 29^ C, gave no perithecia. The lowering of it to 

 the usual room temperature made the same cultures give fruit after 

 twelve or fifteen days. P. fimiseda, wjiich grew in 24^ C. tempe- 

 ratiu-e gaA'e some perithecia. but in a very limited quantity. The 

 rise of temperature, favorably influenced only the vegetative growth 

 of all the three species of Podospora, for their mycelium grew with 

 extraordinary strength and thickness. One cannot say the same 

 for the Sporormia, the growth of which was always very insignifi- 

 cant (see p. loi). By the raising of temperature could be observed 

 quite a different result for the second group, that is P. curvula, 

 P. anserina and Sordaria. This was proved in an accelerated and 

 abundant formation of perithecia. The best results were given 

 by the growth of the fungi in a cupboard A\-ith a constant tempe- 

 rature of 26° С 



The light, which has' generally, such a great influence on the 

 development of fruit bodies of some ascomycetes. for instance Asco- 

 phanus Carneus (Ternetz 1900), had no distinct effect on the for- 

 mation of the perithecia, and the cultures developed normally as 

 well in the dark as in the light. 



Selecting the conditions in winch the cultured fungi developed 

 theii- fruit bodies, one could define that the combination of certain 

 conditions produced the most abundant and the quickest fruiting. 

 Therefore the cultures лvere, generally, made in small Koch's cups 

 with a sterilized mixture, only slightly moistened, of fresh and old 

 horse-dung, or with a slyghtly alkalized IVa^/'o agar-agar with 

 horsedung decoction. These mediums were infected with the myce- 

 lium of the fungus wliich had not been quite cleared of bacteria. 

 The cultures grew in the light, in about 18" C. temperature. And 

 keeping close to all these conditions a splendid result was obtained 

 and one always had at hand, iu an unlimited quantity, material 

 needed for investigation. 



We should only add, that the spores, gathered from the artifi- 

 cial cultures also gerjninated and grew easily, producing normal 

 perithecia, similar to the spores, which had in the beginning ser- 

 ved as the initial material. 



