— 125 — 



then suddenly were brought into a hot cupboard with 29" C. t**. 

 The perithecia after this were fixed consecutively, every half hour. 

 The indicated stage of division was to be seen in the portion that 

 was fixed at the end of 4 hours. Earlier stages than the telephase 

 could not be observed. In the perithecium fixed in 472 ^^ ^ hours 

 could be observed the usual picture, that is to say, in the cells of 

 the ascogonium could be seen only those nuclei, Avhich lie in pairs. 

 The experiment of the artificial retarding of their groAvth was re- 

 peated and the result obtained was identical: that is, great quan- 

 tity of dividing nuclei in the telephase stage in the perithecia, 

 fixed after four hours, and the absence of any stages of division, 

 in later or previously fixed material. 



In cultures, growing in the usual conditions, dividing nuclei did 

 not appear. Does it depend on the specific structure of the nucleus 

 in P. curvula, generally легу poor in chromatine, or on the unu- 

 sual rapidity with which this process proceeded, or are special 

 means of fixing and staining necessary? At any rate one could 

 observe, even such stages of telephase, only after a whole series- 

 of unsuccessful attempts in this direction and only, so to say, in 

 mass dividings of the nuclei. 



Investigations made on the dividing nuclei, in the germinating 

 spores, indicate that in general, one must consider Podospora as a 

 very unfavorable object for the study of the process of dividing. 

 Examining the hyphae developing during the germination of the 

 spores, in which the nuclei attain large dimensions and in which 

 the growth (and relating to same the process of dividing of the 

 nuclei) must be very intense, one could generally ascertain a con- 

 stant disposition of the nuclei in pairs, such as in the ascogonium. 

 Only in quite exceptional cases were found the same figures of 

 dividing, as indicated above, in the cells of the ascogonium ^). 



If from the results obtained one can conclude that the pairing 



1) Vallory also states the complete absence of Avhatever stages of dividing 

 of nuclei in the hyphae of germiuantig spores by Chaetomium Kunzeanum (rela- 

 ted to the Sordariaceae family). He reports, that in a whole series of prepa- 

 ration he investigated, he always met nuclei lying in pairs, which he took for 

 divided in tAvo nuclei. However investigations, which лте made at the same time 

 upon germinating spores on the Chaetomium pannosum (Walhr) showed that 

 from time to time one could meet here same stages of dividing as in P. curvula.- 



